玉米料对叶病的微生物和代谢反应:对发酵和蛋白质稳定性的影响
Liuxing Xu1, Xianfu Lv2, Xiaolu Lu1
1College of Agronomy and Life Sciences, Zhaotong University, Zhaotong, 657000, China.
BMC plant biology
|January 22, 2026
概括
叶状真菌病通过减少蛋白质和碳水化合物而降低玉米料质量. 用Lactiplantibacillus plantarum HT1注射可改善料发酵,并通过减轻疾病影响来保存蛋白质.
科学领域:
- 农业科学 农业科学
- 微生物学 微生物学
- 植物病理学 植物病理学
背景情况:
- 叶子真菌病显著减少植物干物质,对料发酵产生负面影响.
- 玉米中的叶虫害会影响化动力学,微生物的继承和代谢.
- 蛋白质溶解和气损失是病变料的主要问题,影响发酵质量.
研究的目的:
- 研究叶病对玉米料发酵,微生物动力学和代谢的影响.
- 为了检查Lactiplantibacillus plantarum HT1注射是否可以减轻疾病诱导的蛋白质分解.
- 了解由叶病和L. plantarum HT1.1.影响的新陈代谢途径重塑.
主要方法:
- 健康的玉米料 (CON),受疾病影响的料 (DCON) 和受疾病影响的料与L. plantarum HT1 (HT1) 接种的比较.
- 对料发酵参数,微生物种群 (LAB和模具) 和分数 (NH3-N) 的分析.
- 酶活性测定 (氨基酸酶,碳氧酸酶),微生物组分析 (β-多样性) 和代谢学.
主要成果:
- 叶病减少了原始蛋白质和水溶性碳水化合物含量,减少了LAB,并增加了模具.
- 在DCON料中,pH值更高,乳酸更低,黄油酸增加,NH3-N和蛋白质酶活性增加.
- 微生物组分析揭示了DCON的明显分离,具有丰富的蛋白质分解系和上调的氨基酸降解途径.
结论:
- 叶病破坏了的稳态,并放大了蛋白质分解,导致料质量差.
- L. plantarum HT1 注射有效地减少了蛋白质分解和气损失.
- 用L. plantarum HT1的注射恢复了疾病挑战的玉米中的料发酵质量和蛋白质保存.
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