一种绿色的TLC-密度计生物分析方法,用于同时确定duloxetine和risperidone在人体血中与in-silico DDI评估
Fatma Magdy1, Raghda A Emam2, Basma H Anwar3
1Medicinal Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Alshaheed Shehata Ahmad Hegazy St, Beni-Suef, 62514, Egypt.
BMC chemistry
|January 22, 2026
概括
这项研究提出了一种环保的薄层染色学 (TLC) 方法,用于量化人体血中的RISPERIDONE (RSP) 和DLX. 开发的方法是准确的,有选择性的,并符合美国FDA生物分析指南.
科学领域:
- 分析化学 分析化学
- 药理学 药理学是指药理学的学科.
- 环境科学 环境科学
背景情况:
- 精神分裂症的治疗通常涉及与非典型抗精神病药物 (如RISPERIDONE (RSP)) 和抗抑郁药物 (如DLX) 联合治疗.
- 在生物样本中精确量化这些药物对于治疗监测和理解药物相互作用至关重要.
- 在制药分析中,对环境可持续的分析方法的需求越来越大.
研究的目的:
- 开发和验证一种绿色,选择性和准确的薄层染色学 (TLC) 方法,用于同时确定人体血中的DLX和RSP.
- 评估开发的TLC方法的环境影响和分析性能.
- 评估DLX和RSP之间的潜在药物相互作用 (DDI).
主要方法:
- 开发了一种TLC方法,使用甲醇,乙酸盐和氨溶液 (6:4:0.2,v/v) 的溶剂系统.
- 使用230nm的紫外线扫描进行检测,以醇 (PRP) 作为内部标准.
- 方法验证包括使用多种工具进行线性,选择性和环境安全评估,并遵守美国FDA生物分析指南. 使用基于Web的工具进行了DDI分析.
主要成果:
- 通过TLC方法,在注射的人体血中,分别实现了DLX和RSP的明显分离,阻滞系数 (Rf) 为0.32和0.59.
- 在DLX的0.04-0.4μg波段-1和RSP的0.1-0.6μg波段-1之间建立了线性.
- 该方法表现出良好的绿色和蓝色特性,优点数字符合美国FDA的指导方针. DDI分析表明了潜在的相互作用.
结论:
- 开发的TLC方法是一种经过验证,环保,选择性和准确的工具,用于在人体血中量化DLX和RSP.
- 该方法符合监管指南,支持其用于治疗药物监测和药理动力学研究.
- DDI评估提供了关于DLX和RSP联合治疗的安全性和疗效的见解.
相关概念视频
Thin-Layer Chromatography (TLC): Overview
4.5K
Thin-layer chromatography (TLC) is a chromatography technique that separates compounds based on their polarity. TLC typically uses polar silica gel, a form of silicon dioxide, as the stationary phase. The silica gel contains hydroxyl (OH) groups on its surface, which form hydrogen bonds with polar compounds, influencing their adhesion to the stationary phase.
To begin the analysis, a mixture of compounds is spotted on the starting line on the TLC plate using a thin capillary. The bottom of the...
To begin the analysis, a mixture of compounds is spotted on the starting line on the TLC plate using a thin capillary. The bottom of the...
4.5K
Green Algae
743
Green algae, also referred to as chlorophytes, are different from red algae in having the chloroplasts containing chlorophylls a and b, which give them their distinct green hue. However, they lack phycobiliproteins, preventing them from developing the red or blue-green pigmentation seen in red algae. In terms of photosynthetic pigment composition, green algae closely resemble plants and share a close evolutionary relationship with them. Taxonomically Green algae belong to Phylum Chlorophyta in...
743
Protein-Drug Binding: Determination Methods
630
Determining protein-drug binding can be achieved through indirect and direct methods, each providing valuable insights into the interaction between proteins and drugs.
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...
630
Dose Size and Dosing Frequency: Determination Methods
281
Determining the optimal dose size and dosing frequency in pharmacotherapy is crucial for achieving therapeutic effectiveness while minimizing adverse effects. This article explores the methodologies employed in determining these parameters, focusing on their significance and interplay to tailor dosing regimens.Dose Size: Dose size refers to the amount of a drug administered in a single dose. It is determined based on the drug's pharmacodynamics and pharmacokinetics properties and...
281
Dosage Interval and Administration Route: Determination Methods
227
A medication’s effectiveness largely depends on its appropriate dosage and the route of administration. Dosage ensures that a sufficient drug concentration is maintained in the bloodstream to elicit the desired therapeutic effect without causing toxicity. The route of administration affects the drug's bioavailability, rate of absorption, and onset of action, which are crucial for achieving optimal therapeutic outcomes. Drug dosage calculations are critical to tailoring therapy to...
227
Determination of Renal Drug Clearance: Graphical and Midpoint Methods
390
Renal clearance, a crucial parameter in pharmacokinetics, can be determined using two different methods: the graphical method and the midpoint method. These methods provide insights into the rate of drug excretion by the kidneys and aid in assessing renal function.
The graphical method involves plotting the rate of drug excretion in urine against the plasma drug concentration. By analyzing the graph, the clearance can be calculated and obtained. Drugs rapidly excreted by the kidneys exhibit a...
The graphical method involves plotting the rate of drug excretion in urine against the plasma drug concentration. By analyzing the graph, the clearance can be calculated and obtained. Drugs rapidly excreted by the kidneys exhibit a...
390


