时间转录基因图谱揭示了PRRSV感染期间经典和新兴调节细胞死亡途径的顺序参与
Shinuo Cao1, Li Zhang1, Rui Zhu1
1Swine Infectious Diseases Division, Jiangsu Key Laboratory for High-Tech Research and Development of Veterinary Biopharmaceuticals, Engineering Technology Research Center for Modern Animal Science and Novel Veterinary Pharmaceutic Development, Jiangsu Agri-animal Husbandry Vocational College, Taizhou, Jiangsu, China.
Frontiers in veterinary science
|January 22, 2026
概括
猪生殖和呼吸系统综合征病毒 (PRRSV) 感染暂时调节宿主细胞死亡途径. 病毒在早期抑制铁亡,后来激活 cuproptosis 和 disulfidptosis,同时保持免疫细胞死亡信号.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- 猪生殖和呼吸系统综合征病毒 (PRRSV) 由于复杂的免疫病理,在猪业中造成重大经济损失.
- 在PRRSV感染期间控制宿主细胞命运的精确分子机制尚不完全理解.
研究的目的:
- 调查PRRSV感染期间调控细胞死亡 (RCD) 途径的时间动态.
- 阐明PRRSV用于调节宿主细胞死亡和生存的分子策略.
主要方法:
- 在多个时间点 (感染后的0,12,24,36,48,72小时) 进行PRRSV感染的MARC-145细胞的时间转录组分析.
- 对与各种RCD途径相关的基因表达模式的分析,包括铁,,硫化和免疫细胞死亡.
主要成果:
- PRRSV感染显示出RCD通路的复杂时间调节,早期降低ferroptosis的调节和提升细胞保护元素的调节.
- 像cuproptosis和disulfidptosis这样的新兴RCD模式被确定为具有独特的时间激活模式.
- 在整个感染过程中,观察到免疫细胞死亡的持续特征.
结论:
- 通过临时协调的策略,PRRSV广泛调节宿主细胞死亡的转录程序,平衡病毒复制与受控的细胞病理学.
- 这些发现揭示了病毒感染和宿主细胞命运之间的复杂相互作用,为定时治疗干预提供了潜在的目标.
- 了解RCD通路调节的时间分期对于开发有效的策略来缓解PRRSV引起的疾病和增强抗病毒免疫力至关重要.
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