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相关概念视频

The Nitrogen Cycle01:49

The Nitrogen Cycle

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Nitrogen atoms, present in all proteins and DNA, are recycled between abiotic and biotic components of the ecosystem. However, the primary form of nitrogen on Earth is nitrogen gas, which cannot be used by most animals and plants. Thus, nitrogen gas must first be converted into a usable form by nitrogen-fixing bacteria before it can be cycled through other living organisms. The use of nitrogen-containing fertilizers and animal waste products in human agriculture has greatly influenced the...
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Overview of Nitrogen Metabolism01:20

Overview of Nitrogen Metabolism

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Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of  ammonia, ammonium ions, nitrate, nitrite, or  nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
11.0K
Solution Formation02:16

Solution Formation

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There is no one solvent that can dissolve every type of solute. Some substances that readily dissolve in a certain solvent might be insoluble in a different solvent. A simple way to predict which substances dissolve in which solvent is the phrase "like dissolves like". This means that polar substances, such as salt and sugar, dissolve in a polar substance like water. In contrast, non-polar substances are more soluble in non-polar solvents such as carbon tetrachloride.
This selective...
36.9K
Fixation and Sectioning01:03

Fixation and Sectioning

7.5K
Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
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Inorganic Nitrogen Assimilation01:22

Inorganic Nitrogen Assimilation

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Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
493
Intermolecular Forces in Solutions02:28

Intermolecular Forces in Solutions

38.9K
The formation of a solution is an example of a spontaneous process, a process that occurs under specified conditions without energy from some external source.
When the strengths of the intermolecular forces of attraction between solute and solvent species in a solution are no different than those present in the separated components, the solution is formed with no accompanying energy change. Such a solution is called an ideal solution. A mixture of ideal gases (or gases such as helium and argon,...
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相关实验视频

Updated: Jan 23, 2026

Micro-scale Engineering for Cell Biology
04:42

Micro-scale Engineering for Cell Biology

Published on: October 1, 2007

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氧体作为工程生物固化的潜在解决方案

Warren Shou Leong Ang1, August Lipari1, Zhen Guo Oh2

  • 1Boyce Thompson Institute, Ithaca, New York 14853, United States.

ACS synthetic biology
|January 22, 2026
PubMed
概括

研究人员将固酶 (固化必不可少的酶) 改造为细菌微分区,称为碳素体. 该策略旨在保护对氧气敏感的酶,从而有可能在作物中固定,从而实现可持续农业.

关键词:
亚酸酶是一种酸酶.细菌微分区是细菌的微分区.生物固化的生物固.卡尔博克西索姆 (Carboxysome) 是一种蛋白质外是一种蛋白质外.合成生物学 合成生物学

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Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor
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Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

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Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials
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Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

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相关实验视频

Last Updated: Jan 23, 2026

Micro-scale Engineering for Cell Biology
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Micro-scale Engineering for Cell Biology

Published on: October 1, 2007

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Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor
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Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

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Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials
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Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

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科学领域:

  • 生物化学 生物化学
  • 微生物学 微生物学
  • 合成生物学 合成生物学

背景情况:

  • 酶对于生物固定至关重要,它将大气中的转化为氨.
  • 基酶对氧气高度敏感,限制了其在非本土系统中的活性和应用.
  • 碳素体是细菌微分区,自然限制氧气扩散.

研究的目的:

  • 研究在碳素体内对化酶进行细分的可行性,以减轻氧气敏感性.
  • 探索创造"氧体"以增强固化的潜力.
  • 为在作物植物中工程固化的基础奠定基础.

主要方法:

  • 利用基因工程来表达化酶子单元 NifH 融合到碳素酶组向性.
  • 使用电子显微镜可视化工程碳素体的结构.
  • 进行生长实验和质谱测量,以评估蛋白质定位和碳素酶体功能.

主要成果:

  • 成功地实现了 NifH 子单元的选择性局部化到 *Nostoc punctiforme* 碳素体.
  • 电子显微镜证实了充满NifH的碳素体组的正常组合.
  • 质谱测试验证了纯化碳素体内融合蛋白的积累,对碳素体功能的影响最小.

结论:

  • 证明了使用碳素酶体进行细分和保护酶的潜力.
  • 建立了开发含有完全活性酶复合体的"氧体"的基础.
  • 这种方法可以使作物中固化的直接工程成为可能,促进可持续农业和粮食安全.