在ZIF-8/ZnS异构连接上进行电荷转移驱动的反选择性表面增强的拉曼散射:用于定量泌尿乳酸酸酶体过剩的无基拉尔标签生物传感器
Shaorui Qi1, Lei Sun1, Fansheng Meng1
1College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 145040, China.
ACS sensors
|January 22, 2026
概括
一个新的非等离子体SERS平台可以直接量化乳酸 (LA) 反体及其尿液中的多余量 (ee). 这一突破为诊断肠道疾病提供了灵敏,准确和非侵入性的方法.
科学领域:
- 化学传感器 化学传感器
- 生物标志物检测检测 生物标志物检测
- 纳米材料是一种纳米材料.
背景情况:
- 生物流体中异常的乳酸 (LA) 酶体过量 (ee) 与肠道疾病有关.
- 目前用于LA反体分析的方法通常是复杂的,缺乏灵敏度.
- 开发直接的,非侵入性诊断工具对于早期发现疾病至关重要.
研究的目的:
- 开发一种非等离子表面增强拉曼散射 (SERS) 平台,用于直接量化LA反体及其在原始人体尿中的EE值.
- 为了建立一个新的电荷转移驱动的性识别机制,用于enantioselective传感.
- 克服现有的性传感平台的局限性,例如依赖贵金属和复杂的性修饰剂.
主要方法:
- 一个ZIF-8/ZnS异质连接的制造与4-mercaptopyridine (4-MPy) 探针功能化.
- 使用4 - MPy和LA反体之间的电荷转移机制,用于信号放大和奇拉识别.
- 分析SERS光谱和光寿命变化,以定量反体和确定EE.
主要成果:
- 该平台实现了LA反体的超敏感检测极限10nM.
- 证明了对ee值 (R^2 = 0.98) 的线性响应,精度高 (RSD < 8.28%).
- 与酶测定 (RMSEP = 1.97) 和长期稳定性 (28天) 相比,表现出临床级准确性.
结论:
- 开发的ZIF-8/ZnS@4-MPy SERS平台提供了一种高效的,非侵入性的方法,用于分析尿液中的奇拉代谢物.
- 这项工作为无等离子体SERS奇拉传感建立了一个新的方向,适用于各种生物标志物.
- 该技术具有通过尿液分析早期诊断和监测肠道疾病的潜力.
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