从健康和退化的绵羊椎间盘中分离出来的细胞的转录和功能比较
Paul Humbert1, Lucie Danet1, Emmaëlle Carrot1
1Nantes Université, Oniris, CHU Nantes, INSERM, Regenerative Medicine and Skeleton, RMeS, UMR 1229, Nantes, France.
Journal of cellular and molecular medicine
|January 22, 2026
概括
绵羊细胞为研究椎间盘退化 (IVDD) 提供了有价值的模型. 虽然老羊细胞表现出分子退化的迹象,但年轻和老细胞在功能测试中反应相似.
科学领域:
- 生物医学科学 生物医学科学
- 细胞生物学 细胞生物学
- 翻译医学是一种翻译医学.
背景情况:
- 椎间盘退化 (IVDD) 导致慢性腰部疼痛,其机制尚未完全理解.
- 由于与人类的解剖学和生物力学相似性,羊是IVDD的相关模型.
- 使用纤维环 (AF) 和核脉 (NP) 细胞的体外研究可以提供关于与年龄相关的磁盘退化问题的见解.
研究的目的:
- 为了研究羊的AF和NP细胞在体外如何捕捉老化和体内体内的代谢变化.
- 通过将它们的转录资料与人类数据进行比较,评估绵羊盘细胞的翻译相关性.
- 在基底和刺激条件下,评估年轻羊与老羊盘细胞的功能反应.
主要方法:
- 从年轻和老羊的AF和NP细胞进行转录组分析.
- 使用人类单细胞RNA测序数据进行跨物种解卷分析.
- 功能性测试评估细胞对炎症 (IL-1β) 和诱发衰老的刺激的反应.
主要成果:
- 老羊的AF和NP细胞表现出不同的年龄和组织特异的转录特征,包括高调的炎症和衰老相关基因.
- 老羊磁盘中的转录模块与人类退化的磁盘保持一致,支持翻译相关性.
- 尽管存在分子差异,年轻和老年羊细胞在基底和亲退行性条件下表现出相似的功能反应.
结论:
- 绵羊的AF和NP细胞是用于研究IVDD机制和临床前测试的经过验证的体外模型.
- 老羊细胞反映了退化的一些分子方面,但在体外没有比年轻细胞提供额外的功能益处.
- 这项研究验证了IVDD研究的羊模型,突出了人类疾病的保存转录途径.
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