开发一种基于PCB的电化学口味传感器,用于检测 Chikungunya 病毒蛋白质
Syama Sajikumar1, Naveenkumar Sureshkumar1,2, V V R Sai2
1Department of Chemical Engineering, Indian Institute of Technology Madras, Chennai 600036, India. srinivar@iitm.ac.in.
The Analyst
|January 22, 2026
概括
在印刷电路板 (PCB) 平台上的一种新的以aptamer为基础的生物传感器可以检测奇孔古尼亚病毒 (CHIKV) E1蛋白. 这种具有成本效益的电化学传感器在人类血清中检测CHIKV的高灵敏度和特异性.
科学领域:
- 生物医学工程 生物医学工程
- 纳米技术 纳米技术
- 传染病诊断 传染病诊断 传染病诊断
背景情况:
- 奇孔古尼亚病毒 (CHIKV) 感染需要准确和及时的诊断,以防止误诊.
- 目前的诊断方法可能缺乏广泛应用所需的灵敏度,特异性或成本效益.
- 开发新的生物传感器对于快速可靠地检测病毒病原体至关重要.
研究的目的:
- 开发一种基于印刷电路板 (PCB) 的低成本电化学生物传感器,用于检测 Chikungunya 病毒 (CHIKV) E1 蛋白质.
- 创建一个基于aptamer的传感平台,具有高灵敏度和特异性.
- 以检测范围,检测极限和真实样本分析来评估传感器的性能.
主要方法:
- 用于制造需要小样本体积 (∼10μL) 的金涂PCB传感器.
- 为CHIKV E1蛋白质识别开发一种基于阿普坦体的传感策略.
- 使用方波电压测量 (SWV) 进行电化学表征.
- 评估不同度和人类血清样本中的传感器性能.
主要成果:
- 实现了S形校准曲线,显示了从5 pM到100 nM的线性响应.
- 对于CHIKV E1蛋白,确定了10 pM的低检测极限 (LOD).
- 在特异性测试中,传感器对CHIKV E1蛋白具有很高的选择性,在人类血清分析中显示了93%-102%的信号恢复率.
结论:
- 开发的基于PCB的电化学生物传感器是用于敏感和特定的奇孔古尼亚病毒蛋白质检测的有希望的工具.
- 基于aptamer的方法为开发CHIKV.的快速诊断工具提供了一个可行的策略.
- 这种具有成本效益的平台有可能在临床诊断和疾病监测中得到实际应用.
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