中性细胞细胞外陷和DNase在败血症中的活性:与凝血病和器官功能障碍的关联
Yuki Munekata1,2, Mineji Hayakawa3, Akane Shinkai4
1Emergency and Critical Care Center, Hokkaido University Hospital, Sapporo, Japan.
概括
败血症中中性粒细胞外细胞陷 (NET) 成分升高与器官功能障碍和凝血病相关. 然而,脱氧核糖核酶 (DNase) 活性并没有影响本次败血症患者研究中的NET水平或临床结果.
科学领域:
- 免疫学 免疫学 免疫学
- 关键护理医学 关键护理医学
- 生物化学 生化学
背景情况:
- 中性细胞外细胞陷 (NETs) 在先天免疫中至关重要.
- 网络包括无细胞DNA (cfDNA),基因组和HMGB1.
- 失调的NETosis与败血症的发病有关.
研究的目的:
- 为了研究NET成分 (cfDNA,基因组H3,HMGB1) 与脱氧核糖核酶 (DNase) 活性之间的关联.
- 探索NET组件,DNase活动和败血症严重程度,器官功能障碍和凝血病之间的关系.
主要方法:
- 对58名成人败血症患者的回顾性分析.
- 测量血清cfDNA,组织素H3,HMGB1和DNase活动.
- 与顺序性器官衰竭评估 (SOFA) 和散布血管内凝血 (DIC) 评分的相关性分析.
主要成果:
- 更高的cfDNA水平与升高的SOFA和DIC得分,白细胞症和凝血病有关.
- 血清cfDNA,基因素H3和HMGB1水平与DIC严重程度正相关.
- 两组之间没有观察到DNase活性的显著差异,也没有与临床严重程度相关.
结论:
- 严重性败血症中NET成分升高与不良的临床结果有关,反映了NET形成的增加.
- 在败血症患者中,DNase活性似乎没有影响NET水平或临床严重程度.
- NET组件可以作为血严重程度的有价值的生物标志物.
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