后带效应在血清尿侧流抗原测试可能导致弱或假阴性结果
Lieke Ten Have1, Jochem B Buil1,2, Karin van Dijk3
1Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, The Netherlands.
Medical mycology
|January 22, 2026
概括
一个后区效应可以发生在Histoplasma抗原侧向流量试验 (LFA),可能导致虚假阴性. 建议在解释阴性LFA结果时谨慎使用LFA检测结果,特别是当临床怀疑很高时.
科学领域:
- 菌类学 菌类学是指菌类学.
- 免疫诊断 免疫诊断 免疫诊断
- 传染性疾病 传染性疾病
背景情况:
- 血清性质是一种重要的真菌感染,特别是在特有地区.
- 准确和快速的诊断工具对于及时治疗至关重要.
- 侧流检测 (LFA) 提供了检测质体抗原的治疗点选项.
研究的目的:
- 为了研究在质体抗原侧向流量试验 (LFA) 中发生后区效应的发生和影响.
- 为了确定样本稀释是否可以减轻由于后区效应而导致的潜在虚假阴性结果.
主要方法:
- 使用LFA重新检测血清阳性尿样.
- 对未稀释和连续稀释样本的评估.
- 分析LFA结果的差异和潜在的虚假阴性.
主要成果:
- 在12个质体阳性样本中,在6个样本中观察到后区效应.
- 一个样本由于后区效应而在未稀释的形式产生了虚假负结果.
- 两个样本的稀释导致较弱,但仍然是积极的LFA信号.
结论:
- 质体抗原LFA可能会受到后区效应引起的虚假阴性结果的影响.
- 临床医生在解释负面LFA结果时应谨慎行事,特别是在高度怀疑的情况下.
- 进一步验证LFA的表现,包括评估后区现象,是为诊断基质相生症的必要.
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