RNase J 的 GT1 域通过 Arabidopsis 塑体中的 dsRNA 结合来确保 RNA 质量控制
Kevin Baudry1, Sébastien Skiada2,3, Carmit Burstein4
1Boyce Thompson Institute, 533 Tower Rd., Ithaca, NY 14853, United States.
Nucleic acids research
|January 22, 2026
概括
质细胞里核酶J (RNJ) 中的GT1域对于在植物中去除双链RNA (dsRNA) 是至关重要的. 这个域有助于酶局部化和dsRNA结合,防止翻译干扰和化.
科学领域:
- 植物分子生物学 植物分子生物学
- RNA生物学的RNA生物学
- 生物化学 生物化学
背景情况:
- 核糖核酶J (RNJ) 对于叶绿体中的RNA处理至关重要,对于植物发育至关重要.
- 植物中RNJ缺乏导致双链RNA (dsRNA) 的积累,导致翻译抑制和化.
- 陆地植物RNJ具有独特的C端GT1域,通常在DNA结合转录因子中发现.
研究的目的:
- 调查独特的C端GT1域在质体RNase J (RNJ) 功能中的作用.
- 确定GT1域是否对RNJ预防dSRNA积累和相关植物病理的能力至关重要.
主要方法:
- 使用具有修改RNJ版本 (突变/删除GT1域) 的Arabidopsis rnj突变体的补充试验.
- 复合GT1域与dsRNA,DNA和单链核酸的体外结合试验.
- 在体内分析RNJ可溶性和dSRNA水平 (免疫定位,RNA-Seq) 在补充植物.
主要成果:
- 再组合的GT1域在体外结合dRNA和DNA,但不结合单链核酸.
- 缺乏GT1结合能力的RNJ仍然补充了rnj突变表型,但未能防止dsRNA积累.
- GT1域突变影响RNJ在体内溶解度,表明在塑体内酶局部化中的作用.
结论:
- GT1域对RNJ在dRNA去除中的功能至关重要,可能是通过促进酶局部化和/或基质结合.
- GT1域结合dsRNA并影响RNJ定位的能力是维持RNA稳态和防止植物中化的关键.
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