在Magnaporthe oryzae中通过真菌酸盐减少酶促进体替代条件基因表达的协议
Mostafa Rahnama1, Justin King1
1Department of Biology, Tennessee Tech University, Cookeville, TN 38505, USA.
STAR protocols
|January 22, 2026
概括
这项研究引入了一种控制Magnaporthe oryzae中BUF1基因表达的方法,使用一种对有反应的促进物. 这允许基于色素变化的条件基因表达和简单的表型查.
科学领域:
- 分子生物学分子生物学
- 菌类学 菌类学是指菌类学.
- 遗传学 遗传学 是一个
背景情况:
- 控制基因表达对于功能基因组学至关重要.
- 基本或有条件活跃的基因对表达研究提出了挑战.
- 玛格纳波特 (同义词Pyricularia oryzae) 需要精确的遗传工具.
研究的目的:
- 开发一种协议,用于Magnaporthe oryzae中的条件BUF1基因表达.
- 为了实现基因表达的源依赖控制.
- 通过可观测的变化来促进表型选.
主要方法:
- 采用了针对性促进体替代技术.
- 用一种对有反应的促进剂替换了原生BUF1促进剂.
- 生成的Magnaporthe oryzae菌株,具有修改的BUF1基因调节.
主要成果:
- 成功建立了条件的BUF1基因表达.
- 证明了BUF1表达的源依赖控制.
- 观察到明显的色素变化与基因表达水平相关.
结论:
- 开发的协议为M. oryzae.的条件基因表达提供了可靠的方法.
- 这种方法简化了基因研究的表型查.
- 能够进一步对基本基因进行功能性基因组研究.
更多相关视频
09:25Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae
Published on: June 2, 2021
3.3K
10:34Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
Published on: April 14, 2010
16.0K
相关概念视频
What is Gene Expression?
195.1K
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
195.1K
What is Gene Expression?
11.1K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
11.1K
Cell Specific Gene Expression
16.3K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.3K
Cell Specific Gene Expression
5.4K
5.4K
Chromatin Position Affects Gene Expression
24.7K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area.
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
24.7K
mRNA Stability and Gene Expression
6.6K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
6.6K
