用棉花中的IId组WRKY转录因子GhWRKY17进行序列特异性DNA识别的结构基础
Qin Xiao1,2,3, Yu Wang1,2,3, Xinci Shang1,2,3
1Jiangsu Key Laboratory of Drug Discovery and Translational Research for Brain Diseases, College of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu, 215123, China.
The Biochemical journal
|January 22, 2026
概括
WRKY转录因子调节植物生长和应激反应. 这项研究揭示了棉花蛋白 GhWRKY17 的独特 DNA 结合结构,为棉花纤维发育调节提供了洞察力.
科学领域:
- 植物分子生物学 植物分子生物学
- 结构生物学是结构生物学.
- 遗传学 是一个遗传学.
背景情况:
- WRKY转录因子对于植物发育和应激反应至关重要.
- 在Gossypium hirsutum中的GhWRKY17通过W-box结合来调节棉纤维的发育.
- 组IId WRKY DNA识别的结构基础以前是未知的.
研究的目的:
- 确定GhWRKY17 WRKY域与GhHOX3促进体DNA结合的晶体结构.
- 阐明底层的分子机制IId组 WRKY DNA识别特异性.
- 为工程棉纤维特征提供见解.
主要方法:
- 在1.8 Å分辨率的X射线晶体学.
- 对GhWRKY17 WRKY域-DNA复合物的结构分析.
- 与其他WRKY-DNA复合体进行结构比较分析.
主要成果:
- 确定了与GhHOX3促进体DNA结合的GhWRKY17 WRKY域的晶体结构.
- GhWRKY17采用了一种涉及β2和β3链的双链合机制.
- 由关键残留物形成的特定的结网识别了扩展的G/TTTGACC图案.
- 与其他子组相比,IId组WRKY蛋白具有明显的DNA结合特征.
结论:
- 这项研究提供了第一个IId组WRKY蛋白质-DNA复合物的结构特征.
- GhWRKY17独特的DNA结合机制使其与其他WRKY亚组区别开来.
- 这些发现为IId组WRKY函数建立了一个结构范式,并为棉纤维特征工程提供了洞察力.
更多相关视频
09:17Structure-Based Simulation and Sampling of Transcription Factor Protein Movements along DNA from Atomic-Scale Stepping to Coarse-Grained Diffusion
Published on: March 1, 2022
3.6K
11:25Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences
Published on: February 11, 2019
8.5K
相关概念视频
02:16Transcription Factors
82.3K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
82.3K
02:35Transcription Elongation Factors
13.5K
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
13.5K
02:35Transcription Elongation Factors
4.6K
4.6K
01:30General Transcription Factors
6.8K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
6.8K
01:10Transcription
155.8K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
155.8K
01:52Eukaryotic Transcription Inhibitors
10.9K
Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
10.9K