PRRSV核和非结构蛋白通过ATF-2和AP-1通路双重调节CD40表达
Chaolun Fu1, Hongyan Gao1, Yingchao Li1
1College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China.
猪生殖和呼吸系统综合征病毒 (PRRSV) 改变了巨细胞CD40表达. PRRSV核体蛋白调高CD40,而其他病毒蛋白调低CD40,提供潜在的抗病毒标.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 分子生物学分子生物学
背景情况:
- 巨细胞表面分子对于调节炎症反应至关重要.
- 猪生殖和呼吸系统综合征病毒 (PRRSV) 感染猪膜巨细胞,改变宿主表面受体表达.
- 了解PRRSV对巨细胞受体的调节是控制感染的关键.
研究的目的:
- 为了研究PRRSV感染期间巨细胞上免疫调节分子表达的变化.
- 为了确定参与调节CD40表达的特定PRRSV蛋白质.
- 探索CD40调制对PRRSV复制的功能后果.
主要方法:
- 在感染PRRSV的巨细胞中查免疫调节分子表达.
- 报告员测试评估病毒蛋白对CD40促进体活性的影响.
- 在CD40调控中对转录因子参与 (ATF-2,AP-1) 的分析.
主要成果:
- CD40被确定为PRRSV感染的巨细胞中最显著上调的转录.
- PRRSV核蛋白 (N) 通过ATF-2增强了CD40促进体活性.
- 通过AP-1,PRRSV非结构性蛋白Nsp1α,Nsp11和Nsp12通过AP-1抑制了CD40促进体活性.
- 过度表达CD40限制了PRRSV的复制.
结论:
- 通过其核体和非结构性蛋白质,PRRSV通过差异调节CD40表达.
- CD40在限制PRRSV复制方面发挥着作用.
- CD40代表了针对PRRSV的抗病毒策略的潜在治疗标.
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