菌毒素脱氧尼瓦诺通过细胞壁重塑驱动Bacillus cereus的抗生素耐药性演变,从而危及临床关键的抗生素
Fengru Deng1, Linyu Ke1, Mi Huang1
1State Key Laboratory of Swine and Poultry Breeding Industry, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong 510642, P. R. China.
Journal of hazardous materials
|January 22, 2026
概括
益生菌中脱氧尼瓦伦醇 (DON) 的污染会通过改变细胞壁来驱动像Bacillus cereus这样的细菌的抗生素耐药性. 这种菌毒素暴露也增加了肠道微生物群中的毒性因子和抵抗基因.
科学领域:
- 微生物学 微生物学
- 食品安全 食品安全
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 脱氧尼瓦伦醇 (DON) 是食品和料中普遍存在的真菌毒素污染物.
- 益生菌产品缺乏针对DON的专用监测标准,造成公共卫生风险.
- DON诱导的细菌抗生素耐药性的机制在很大程度上是未知的.
研究的目的:
- 阐明DON诱导Bacillus cereus抗生素耐药性的分子机制.
- 调查含有Bacillus的益生菌产品中DON污染的流行情况.
- 评估DON暴露对肠道微生物群和体内抗生素耐药性的影响.
主要方法:
- 对30种益生菌产品中DON污染的分析.
- 对益生菌产品和孤立的B. cereus菌株进行了次致命DON暴露实验.
- 评估抗生素耐药性 (MICs),基因表达 (细胞壁重塑),DNA突变和肠道微生物群转移在DON暴露的猪.
主要成果:
- 在所有分析的益生菌产品中检测到普遍的DON污染.
- 在B. cereus.中,次致命的DON暴露诱导了对最后避难抗生素 (万科米辛,达普托米辛) 的高水平耐药性.
- DON引发了ROS爆发,DNA突变,以及高调的细胞壁重塑基因,包括lrgA,从而产生了广泛的抗生素耐药性.
- 在体内对猪进行的研究表明,DON诱导的肠道失生症,毒性因子的丰富,以及增加的万科米辛抗性基因.
结论:
- 毒素污染的益生菌是细菌抗生素耐药性和毒性的未被认可的驱动因素.
- DON暴露促进了整个生态系统对最后手段抗生素的耐药性趋同演变.
- 了解DON的机制对于减轻抗微生物药物耐药性的传播至关重要.
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