使用LC-MS/MS和基于特征的分子网络研究酒副产品trub的多步骤策略,评估其抗氧化潜力
Paolo Scognamiglio1, Vittorio Carlucci2, Nadia Benedetto2
1TheBlueChemistryLab Group, Department of Pharmacy, Università degli Studi di Napoli Federico II, Napoli, Italy.
Food chemistry
|January 22, 2026
概括
酒的副产品trub含有众多的生物活性化合物. 一项新的战略确定了新的蜂苦酸类似物和抗氧化剂潜力,支持废物再循环应用.
科学领域:
- 生物技术是生物技术.
- 自然产品化学 自然产品化学
- 分析化学 分析化学
背景情况:
- 造废弃物流Trub富含代谢物,但尚未得到充分的探索.
- 特鲁布中的生物活性化合物为营养药和制药应用提供了潜力.
- 需要有效的方法来描述复杂的生物废物流.
研究的目的:
- 开发和应用生物勘探问题多步骤战略.
- 为了识别和描述trub.内的生物活性代谢物.
- 评估trub在可持续废物回收利用方面的潜力.
主要方法:
- 详尽的提取和液态染色学-高分辨率质谱/质谱 (LC-HRMS/MS) 分析.
- 基于特征的分子网络 (FBMN) 用于代谢物去复制.
- 无监督的多变量分析和生物活性分析 (抗氧化剂测定).
主要成果:
- 在乙醇提取物中识别了大约700种集群代谢物.
- 18个α-和β-酸类比候选人的假定注释,扩大已知的蜂苦酸多样性.
- 通过多变量分析观察到与特定代谢物相关的抗氧化潜力.
结论:
- 综合的多步骤策略使废物流中的化学多样性能够快速和广泛地评估.
- 特鲁布是新型生物活性化合物的宝贵来源,包括蜂苦酸类似物.
- 这种方法支持营养药品和制药行业的可持续废物回收利用.
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