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医院废水环境分离物中的等离子体传播的多药耐药性基因:对耐药性传播的影响

Prajakti¹, Manish Kumar Pandey², Kunal Mukhopadhyay¹

¹Department of Bioengineering and Biotechnology, Birla Institute of Technology, Mesra, Ranchi, 835215, Jharkhand, India.

The Science of the total environment

|January 22, 2026

概括

环境细菌在等离子体上携带抗生素耐药性基因 (ARG),构成公共卫生威胁. 这项研究将ARG映射到来自医院污染水的等离子体上,证实了它们在传播抗生素耐药性 (AR) 中的作用.

科学领域:

环境微生物学环境微生物学
基因组学就是基因组学.
公共卫生公共卫生

背景情况:

环境细菌中抗生素耐药性 (AR) 是一个日益严重的公共卫生问题.
移动遗传元素,特别是等离子体,是抗生素耐药性基因 (ARG) 传播的关键驱动因素.
之前的工作已经确定了多药耐药的Aeromonas spp. 在受医院废水影响的水生环境中.

研究的目的:

从环境细菌中重建和分析等离子体序列.
在这些等离子体上识别和绘制抗生素耐药性基因 (ARG).
了解塑体在水生环境中ARG传播中的作用.

主要方法:

使用等离子体SPAdes.des的等离子体序列重建.
使用综合抗生素耐药性数据库 (CARD) 进行ARG的in silico识别.
对ARG分布进行网络分析,然后进行PCR,测序和定量PCR (qPCR) 验证.

主要成果:

重建了五个等离子体序列,揭示了不同的ARG.
网络分析确定了所有等离子体共同的ARG和独特的ARG.
实验验证证证实了在等离子体上存在并量化了关键ARG的丰度.

结论:

质粒在环境细菌中ARG的传播中发挥着重要作用.
结合in silico和实验方法的综合方法为AR监测提供了一个强大的框架.
这些发现凸显了需要在环境环境中全面监测等离子体传播的ARG.

关键词:

绝对的qPCR是什么航空公司Aeromonas 抗生素耐药性基因是抗生素耐药性的基因.抗生素耐药细菌是抗生素耐药的细菌.医院的废水医院的废水网络网络网络网络网络网络塑体塑体塑体

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