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相关概念视频

Carbohydrate Digestion00:57

Carbohydrate Digestion

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Carbohydrate digestion and metabolism break down simple and complex carbohydrates from food into saccharides (i.e., sugars) for the body to use as energy. Carbohydrate digestion starts in the mouth during mastication, or chewing. The masticated carbohydrates remain intact in the stomach. Digestion resumes in the duodenum of the small intestine, where pancreatic alpha-amylase and brush border enzymes of the microvilli convert complex carbohydrates to monosaccharides. Finally, the monosaccharides...
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What is Monogastric Digestion?01:50

What is Monogastric Digestion?

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The human body contains a monogastric digestive system. In a monogastric digestive system, the stomach only contains one chamber in which it digests food. Several other animal species also have monogastric digestive systems, including pigs, horses, dogs, and birds. This chapter, however, focuses on the human digestive system.
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Lipid Digestion01:06

Lipid Digestion

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Lipids are large molecules that are generally not water-soluble. Since most of the digestive enzymes in the human body are water-based, there are specific steps the body must take to break down lipids and make them available for use.
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Protein Digestion01:02

Protein Digestion

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Protein digestion begins in the stomach, where the highly acidic environment can easily disrupt protein structure by exposing the peptide bonds of polypeptide chains. After polypeptide chains are broken into individual amino acids by a series of digestive enzymes, the amino acids are transported to the liver via the bloodstream to produce energy.
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Regulation of the Digestive System01:25

Regulation of the Digestive System

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Digestive activity regulation hinges on three primary components. Activation is prompted by a multitude of mechanical and chemical indicators, primarily detected by receptors within the stomach and intestines' walls. These receptors predominantly respond to factors such as mechanical stretching of the organ walls, changes in pH and osmolarity, and the presence of digesting materials and their by-products.
The effectors in this regulation system are glands and smooth muscles. Activation of...
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Other Disorders of Digestive System01:30

Other Disorders of Digestive System

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The gastrointestinal tract is susceptible to various disorders. If the lower esophageal sphincter is damaged, stomach acid can flow back into the esophagus, causing irritation and inflammation of the lining. This condition is called gastroesophageal reflux disease (known as heartburn) and may cause chest pain and difficulty swallowing. In the stomach, prolonged use of nonsteroidal anti-inflammatory drugs like aspirin, chronic alcohol consumption, bacterial infections such as Helicobacter...
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快速高通量抗体分析使用微波辅助消化.

Thais Mingatos de Toledo1, Hellen Paula Valerio1, Antônio Moreira Marques Neto2

  • 1Laboratory of Glycoproteomics, Parasitology Department, Biomedical Science Institute, São Paulo University, São Paulo, Brazil.

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概括

微波辅助消化与质谱学相结合,提供了一种快速,可靠的抗体分析方法,显著减少了除化,并改善了用于全面表征的鉴定.

关键词:
抗体是一种抗体.取决于数据的获取采集.数据独立的获取获取数据.微波辅助消化器的消化方式后翻译修改后的修改.

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科学领域:

  • 生物化学 生物化学
  • 分析化学 分析化学
  • 蛋白质科学 蛋白质科学

背景情况:

  • 单克隆抗体是关键的生物治疗药物,需要严格的质量控制.
  • 使用质谱的多属性方法 (MAM) 对于评估关键质量属性 (CQA) 至关重要.
  • 传统的蛋白质消化方法可能是耗时的,并引入文物.

研究的目的:

  • 开发和优化抗体样本的快速微波辅助 (MW) 消化工作流程.
  • 评估不同缓冲区和pH对MW消化效率和人工物形成的影响.
  • 为了比较数据依赖获取 (DDA) 和数据独立获取 (DIA) 进行全面的抗体表征.

主要方法:

  • 开发一个高通量 (96样本) 质谱工作流程.
  • 在各种缓冲条件和pH下评估微波辅助蛋白质消化.
  • 使用DDA和DIA进行分析,加上和翻译后修饰 (PTM) 探测.

主要成果:

  • MW辅助消化提供了快速,可靠的蛋白质消化,具有高序列覆盖率和最小化文物.
  • 与DDA相比,DIA结合MW消化增强了的识别和PTM检测.
  • 在MW条件下,酸缓冲剂在减少除化和氧化方面最有效.

结论:

  • MW辅助消化是一种优越的替代方法,比传统的抗体样本制备方法更好.
  • 优化的MW消化工作流与DIA相结合,可实现全面而公正的抗体表征.
  • 为了解决蛋白质氧化问题,需要进一步优化,但该方法显著减少了其他人工物,如脱化.