用乳糖利用酵母物种发酵水性冰淋副产品的发酵
Amanda L Miller1, John A Renye1, Jennifer Thomas2
1Dairy and Functional Foods Research Unit, USDA Agricultural Research Services, Wyndmoor, PA 19038.
Journal of dairy science
|January 22, 2026
概括
这项研究探讨了用于乙醇生产的废冰淋副产品的酵母发酵. 麦片菌 (Saccharomyces cerevisiae) 显示出最高的乙醇产量,因此非常适合利用这种废物流.
科学领域:
- 生物技术是生物技术.
- 微生物学 微生物学
- 可持续化学 可持续化学
背景情况:
- 废冰淋 (WIC) 副产品是可发酵糖的潜在来源.
- 酵母菌株在发酵乳糖和糖糖的能力上有所不同,乳糖和糖糖是WIC的关键成分.
研究的目的:
- 评估不同酵母菌株在乙醇生产的WIC副产品上的发酵性能.
- 为了比较使用乳糖的酵母 (Kluyveromyces marxianus,Dekkera anomala,Kluyveromyces lactis) 与不使用乳糖的酵母 (Saccharomyces cerevisiae) 的乙醇产量.
- 评估乳糖处理和糖成分对发酵效率的影响.
主要方法:
- 使用四种酵母菌株 (K. marxianus Y-329,D. anomala Y-1414,K. lactis 8585,S. cerevisiae Y-45) 进行发酵试验,并使用WIC副产品.
- 通过减肥监测发酵进展,并使用高性能液态染色学 (HPLC) 分析发酵成分.
- 在最小介质中研究糖的利用 (乳糖,糖糖) 和乳糖预处理的效果.
主要成果:
- 麦芽菌 (Saccharomyces cerevisiae) 从WIC副产品中获得了最高的乙醇产量 (>10%,在72小时达到12.5%),特别是在乳糖处理后.
- 其他测试的酵母显示出较低的乙醇产量,其中一些由糖糖抑制 (降解物抑制) 或受到乙醇耐受性的限制.
- 所有菌株都利用了糖,而对于D. anomala和K. lactis,只有在缺乏糖的情况下才观察到乳糖的利用.
结论:
- 细菌菌是最适合的酵母菌株,用于将废弃的冰淋副产品转化为乙醇.
- 乳糖酶预处理增加了可发酵碳水化合物的可用性,大大提高了乙醇产量.
- 了解糖代谢和潜在的抑制因素,如催化剂抑制,对于优化WIC生物乙醇生产至关重要.
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