Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Introduction to Membrane Proteins01:16

Introduction to Membrane Proteins

80.8K
The cell membrane, or plasma membrane, is an ever-changing landscape. It is described as a fluid mosaic where various macromolecules are embedded in the phospholipid bilayer. Among the macromolecules are proteins. The protein content varies across cell types. For example, mitochondrial inner membranes contain ~76% protein content, while myelin contains ~18% protein content. Individual cells contain many types of membrane proteins—red blood cells contain over 50—and different cell...
80.8K
Membrane Proteins01:30

Membrane Proteins

29.3K
Plasma membranes have integral transmembrane proteins involved in facilitated transport. These proteins are collectively referred to as transport proteins, and they function as either channels for the material or as carriers themselves. Channel proteins have hydrophilic domains exposed to the intracellular and extracellular fluids and a hydrophilic channel through their core that provides a hydrated opening for solutes to pass through the membrane layers. Passage through the channel allows...
29.3K
Colors and Magnetism03:02

Colors and Magnetism

14.0K
Color in Coordination Complexes
When atoms or molecules absorb light at the proper frequency, their electrons are excited to higher-energy orbitals. For many main group atoms and molecules, the absorbed photons are in the ultraviolet range of the electromagnetic spectrum, which cannot be detected by the human eye. For coordination compounds, the energy difference between the d orbitals often allows photons in the visible range to be absorbed and emitted, which is seen as colors by the human...
14.0K
Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

5.5K
Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
5.5K
Color Vision01:24

Color Vision

1.4K
Color perception begins in the retina, the light-sensitive layer at the back of the eye. Two main theories explain how colors are seen: the trichromatic theory and the opponent-process theory. The trichromatic theory, proposed by Thomas Young in 1802 and extended by Hermann von Helmholtz in 1852, suggests that color vision is based on three types of cone receptors in the retina. These cones are sensitive to different but overlapping ranges of wavelengths corresponding to red, blue, and green.
1.4K
Detergent Purification of Membrane Proteins01:18

Detergent Purification of Membrane Proteins

6.3K
Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
6.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Done EAZY: An Automated Procedure for <sup>89</sup>Zr-Radiolabeling and Size-Exclusion Chromatography Purification of Nanoliposomal Anticancer Therapeutics.

Molecular pharmaceutics·2026
Same author

Predicting Clinical Sensitivities of PDGFRA Exon 18 Mutations to Imatinib and Avapritinib to Optimize Gastrointestinal Stromal Tumor Treatment.

Cancer research communications·2026
Same author

HDAC7 controls anti-viral and anti-tumor immunity by CD8<sup>+</sup> T cells.

Frontiers in immunology·2026
Same author

Multi-layered molecular profiling informs the diagnosis and targeted therapy of desmoplastic small round cell tumor.

Nature communications·2026
Same author

MyCODE: a prospective evaluation of lay-language molecular tumor board protocols in precision oncology.

The oncologist·2026
Same author

Elimusertib enhances cytotoxic effects of conventional chemotherapy and sensitizes to radiation in preclinical Ewing sarcoma models.

Scientific reports·2026

相关实验视频

Updated: Jan 24, 2026

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

9.0K

膜蛋白的双色扩展显微镜使用生物直角标记.

Steven Edwards1, Birthe Meineke2, Sebastian Bauer2

  • 1Science for Life Laboratory, KTH Royal Institute of Technology, 171 21 Solna, Sweden.

Nano letters
|January 22, 2026
PubMed
概括

这项研究引入了一种结合非正规氨基酸 (ncAA) 标记和扩展显微镜 (ExM) 的新方法,用于精确,高分辨率的生物成像. 这种方法克服了光显微镜的局限性,使细胞结构的纳米尺度可视化成为可能.

关键词:
生物对等化学的化学.扩展显微镜扩展显微镜连接错误是因为连接错误.非正规氨基酸的氨基酸.特定地点的标签.超高分辨率显微镜的使用方法

更多相关视频

Label-Retention Expansion Microscopy LR-ExM Enables Super-Resolution Imaging and High-Efficiency Labeling
07:44

Label-Retention Expansion Microscopy LR-ExM Enables Super-Resolution Imaging and High-Efficiency Labeling

Published on: October 11, 2022

4.3K
Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes
10:25

Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes

Published on: September 27, 2024

1.1K

相关实验视频

Last Updated: Jan 24, 2026

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

9.0K
Label-Retention Expansion Microscopy LR-ExM Enables Super-Resolution Imaging and High-Efficiency Labeling
07:44

Label-Retention Expansion Microscopy LR-ExM Enables Super-Resolution Imaging and High-Efficiency Labeling

Published on: October 11, 2022

4.3K
Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes
10:25

Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes

Published on: September 27, 2024

1.1K

科学领域:

  • 细胞生物学 细胞生物学
  • 显微镜技术 显微镜技术
  • 生物化学 生物化学

背景情况:

  • 光显微镜的分辨率通常受到标签大小和链接错误的限制,而不是显微镜.
  • 基于抗体的探针在光蛋白标记中引入了空间的不确定性.
  • 与生物直角点击化学相结合的特定地点的ncAA结合提供了更好的标签精度.

研究的目的:

  • 开发和验证一种结合ncAA标记和扩展显微镜 (ExM) 的方法,用于双色超高分辨率成像.
  • 为了提高标记精度,超越目前基于抗体的方法.
  • 为了实现特定蛋白质子单元的纳米尺度可视化.

主要方法:

  • 非正规氨基酸 (ncAAs) 在蛋白质中进行特定地点的结合.
  • 使用生物直角点击化学进行光标签.
  • 应用扩展显微镜 (ExM) 来增加样本大小并提高分辨率.
  • 进行超高分辨率的STED成像用于验证.

主要成果:

  • 实现了优化ncAA标签程序和光体选择.
  • 在扩展的HEK 293T细胞中成功可视化和解决Na,K-ATPase α1和β1亚单元的纳米分布.
  • 在未扩展的ncAA标记细胞上使用STED成像验证了该方法.

结论:

  • 结合ncAA标签和ExM,为多重复,高分辨率成像提供了一个强大的框架.
  • 这种方法使纳米尺度的生物成像成为可能,克服了以前的分辨率限制.
  • 开发的技术显著提高了超分辨率显微镜的标记精度.