粘膜DNA甲基化揭示了与免疫相关的甲基化特征,与克罗恩病状态相关
Tianyu Zhang1, Qiaowen Lin1, Wang-Yang Xu2
1Department of Gastroenterology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin 2nd Road, Shanghai, 200025, China.
Scientific reports
|January 22, 2026
概括
在中国克罗恩病中DNA甲基化模式.
科学领域:
- 表观遗传学和胃肠道学
背景情况:
- 改变的DNA甲基化 (DNAm) 与克罗恩病 (CD) 病原发生有关.
- 在中国CD患者中DNAm的作用及其与疾病状况的相关性尚不清楚.
研究的目的:
- 系统地调查中国CD患者的DNAm模式.
- 为潜在的诊断和预后应用,将这些DNAm模式与疾病状态联系起来.
主要方法:
- 减少代表性双硫酸盐测序 (RRBS) 在24名未接受过治疗的成年CD患者的炎症和非炎症肠粘膜上进行.
- 不同甲基化分析确定了炎症特异性和疾病严重程度特异性的特征.
主要成果:
- 在炎症的粘膜中发现了17,097个差异甲基化位点 (DMC) 和2,687个差异甲基化区域 (DMR).
- 与炎症相关的DMRs在免疫细胞功能和细胞粘附途径上得到了丰富.
- 特定的DMR与疾病严重性得分 (SES-CD和CDAI) 相关联,其中六个基因 (KDM4B,CLDN15,PGGHG,SLC25A10,KIAA2013,N4BP1) 与炎症和疾病活动有显著关联.
结论:
- DNA甲基化反映了克罗恩病患者的免疫性肠道变化.
- DNAm 档案可以根据疾病严重程度区分患者,这表明它有可能作为疾病管理的预测生物标志物.
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