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压力增强的双固体表面超快速冷却改善了肝细胞和精确切割肝脏切片的解后恢复
Mohammad Amini1,2, James D Benson3
1Department of Mechanical and Mechatronics Engineering, Memorial University of Newfoundland, St. John's, A1B 3X5, Canada.
Scientific reports
|January 22, 2026
概括
高压双面冷却通过增加冷却速率并最大限度地减少冰晶的形成,显著提高了细胞和组织的冷保存. 这种方法可以提高生物材料的解后活力.
科学领域:
- 生物医学工程 生物医学工程
- 低温生物学 低温生物学
- 细胞生物学 细胞生物学
背景情况:
- 冷保存对于生物材料的分布至关重要,但受到冰晶形成的限制.
- 快速冷却和降低冷剂 (CPA) 度是缓解冰损伤和细胞毒性的关键.
- 高压可以改变溶液特性 (化温度,核化温度,玻璃过渡温度),有利于玻璃化.
研究的目的:
- 设计和测试一种用于密封生物样品的高压双面冷却的新型装置.
- 与传统技术相比,评估这种方法在改善冷保存结果方面的有效性.
- 为了确定最佳的压力和CPA度,以提高组织活力.
主要方法:
- 开发和制造了一种用于密封样品的高压双面冷却装置.
- 使用二甲基硫氧化物 (DMSO) 作为CPA,度为10-30%.
- 在HepG2细胞和小鼠肝切片 (PCLS) 的快速冷却过程中,施加的压力从50到200MPa不等.
主要成果:
- 双面,高压导体冷却实现了比对流方法 (例如,液浸泡) 更高的冷却速度.
- 在20%的DMSO和150MPa下观察到最佳的冷保存结果,即最高的解后活力.
- 高压系统有效地抑制了冰晶的形成,改善了玻璃化.
结论:
- 高压双面冷却是快速冷保存细胞和组织的优越方法.
- 这种技术提供了一个有前途的策略,以提高生物材料的全球分布,降低CPA毒性.
- 进一步的研究可以优化各种细胞和组织类型的压力和CPA组合.
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