一个模块化的单基因和双基因表达工具包,用于Kluyveromyces marxianus
Zewei Lu1, Changhui Lv1, Zhuoer Chen1
1Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.
World journal of microbiology & biotechnology
|January 22, 2026
概括
我们为Kluyveromyces marxianus开发了新的基因表达系统,Kluyveromyces marxianus是一种用于合成生物学的酵母. 这些工具改善了蛋白质的生产,使这种酵母成为工业应用中更具多功能性的底盘.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 合成生物学 合成生物学
背景情况:
- Kluyveromyces marxianus是一种强大的酵母,具有工业应用的潜力.
- 在K. marxianus中,有限的基因表达工具阻碍了其在合成生物学中的应用.
- 开发高效的表达系统对于扩大其应用至关重要.
研究的目的:
- 开发用于Kluyveromyces marxianus的新型单基因和双基因表达系统.
- 根据选的遗传元素,碳源和培养条件来优化基因表达.
- 为了增强蛋白质的生产,并使K. marxianus.中的亚细胞向.
主要方法:
- 查和促进体 (pENO,pHTX) 和基因间序列的开发.
- 在多个K. marxianus菌株中构建和测试单基因和双基因表达系统.
- 通过不同的碳来源 (银糖,葡萄糖) 和培养时间来优化表达.
- 使用 Simian Virus 40 (SV40) 核定位信号 (NLS) 进行亚细胞向.
主要成果:
- 在不同的K. marxianus菌株中实现了稳定的基因表达.
- 单基因系统与双基因表达产生了30.4 mg/L的黑血球蛋白 (VaHB) 在银河糖上.
- 双基因系统共同表达HEM1,在葡萄糖上达到48.4 mg/L的VaHB.
- SV40 NLS成功地将光蛋白导向到细胞核.
结论:
- 开发的工具包为K. marxianus.提供了高效和响应环境的基因表达.
- 这些系统增强了K. marxianus作为工业蛋白质生产的微生物底盘.
- 这项研究为K. marxianus.提供了更广泛的合成生物学应用.
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