低成本,手持式,多脉冲电穿孔器,用于简化在皮肤中输送核酸
Pankaj Rohilla1, Erkan Azizoglu1, Sion Park1
1School of Chemical & Biomolecular Engineering Georgia Institute of Technology Atlanta Georgia USA.
Bioengineering & translational medicine
|January 23, 2026
概括
一种名为RotoPatch的新型手持设备使用微针来通过电穿孔有效地通过皮肤内输送核酸. 这项技术为基因疗法和疫苗接种应用提供了低成本,便携式的替代方案.
科学领域:
- 生物医学工程 生物医学工程
- 基因治疗 基因治疗
- 疫苗学 疫苗学 疫苗学
背景情况:
- 基于载体的核酸输送 (脂质纳米颗粒,病毒载体) 面临挑战,包括体内稳定性差,免疫性,毒性和非目标效应.
- 传统的电孔装置往往是昂贵的,重的,缺乏便携性,需要痛苦的管理.
研究的目的:
- 开发一种新的,小型的,低成本的,手持式压电电穿孔器,用于皮肤内核酸输送.
- 评估RotoPatch设备对细胞内核酸的吸收和体内核酸表达的疗效.
主要方法:
- 开发了RotoPatch系列使用微针电极进行皮肤内输送的压电电解器.
- 通过单个旋转动作给予多个电穿孔脉冲,将电场定位到皮肤的上层.
- 用生物发光和光成像对小鼠和老鼠的mRNA和等离子体DNA细胞内吸收的评估.
主要成果:
- 在小鼠中,RotoPatch显示火 luciferase 编码的mRNA 的细胞内吸收增强.
- 在老鼠中,RotoPatch促进了绿色光蛋白编码的等离子体DNA的更大吸收.
- 通过RotoPatch获得的体内表达水平与现有的多脉冲电穿孔装置 (ePatch,eIgniter) 相当.
结论:
- 多脉冲压电微针电穿孔,如RotoPatch所示,是用于皮肤内核酸输送的可行平台.
- 这项技术在推进基因疗法和疫苗接种策略方面具有重大潜力.
- 该RotoPatch提供了一个便携式,具有成本效益,并可能比传统的电穿孔方法更轻松的替代方案.
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