化碳量子点在体外受精和早期胚胎发育中通过改善小鼠的精子活化来提高胚胎发育
Hyunhee Song1,2, Jae-Wan Jung2, Hoon Jang1,2
1Department of Life Science, Jeonbuk National University, Jeonju 54896, Korea.
Development & reproduction
|January 23, 2026
概括
在低剂量中,化碳量子点 (NCQD) 增强精子功能和早期胚胎发育. 然而,更高的暴露抑制了后来的胚胎分化,表明了特定阶段的影响.
科学领域:
- 生殖生物学 生殖生物学
- 纳米技术 纳米技术
- 发展生物学 发展生物学
背景情况:
- 添加碳量子点 (NCQD) 具有独特的光学特性和潜在的生物活性.
- 它们对生殖细胞和早期胚胎发育的影响在很大程度上是未知的.
研究的目的:
- 研究NCQD对小鼠的精子功能,受精和体外胚胎发育的影响.
- 了解NCQD对生殖结果的剂量依赖和特定阶段的影响.
主要方法:
- 在体外研究中使用了小鼠模型.
- 使用计算机辅助精子分析 (CASA) 评估精子参数 (运动性,存活率,容量).
- 评估了受精率,早期胚胎发育,胚胎细胞形成和基因表达 (Oct4,Cdx2,Ade2,Atf6,Chop).
主要成果:
- 低度 (10微克/毫升) 的NCQD改善了精子运动性,存活率和容量,增加了受精率.
- 提升了早期胚胎发育到Morula阶段的增强.
- 延迟胚胎囊形成和减少多能性和 trofhectoderm 标志物.
- 在毛囊胚胎的胚胎中增加了内等质网膜压力标志物.
结论:
- NCQDs表现出双相效应:促进精子激活和早期分裂,但抑制后来的分化.
- 长时间的NCQD暴露可以诱导细胞应激,可能会干扰血统规范.
- 优化NCQD剂量和暴露时间对于在生殖环境中安全应用至关重要.
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