相关实验视频

Updated: Jan 24, 2026

CRISPR Guide RNA Cloning for Mammalian Systems
06:48

CRISPR Guide RNA Cloning for Mammalian Systems

Published on: October 2, 2018

72.9K

一个引导RNA重复检查点引导CRISPR-Cas9催化.

Ramadevi Chilamkurthy, Sruthi Sudhakar, Adrian A Pater

    bioRxiv : the preprint server for biology
    |January 23, 2026
    PubMed
    概括

    这项研究揭示了一个指导RNA重复检查点 (GRC),它调节了CRISPR-Cas9基因编辑. 这一发现使得改进基因编辑工具的开发成为可能,并减少了非目标效应.

    更多相关视频

    Generation of Brown Fat-Specific Knockout Mice Using a Combined Cre-LoxP, CRISPR-Cas9, and Adeno-Associated Virus Single-Guide RNA System03:58

    Generation of Brown Fat-Specific Knockout Mice Using a Combined Cre-LoxP, CRISPR-Cas9, and Adeno-Associated Virus Single-Guide RNA System

    Published on: March 24, 2023

    3.1K
    Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays11:05

    Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays

    Published on: January 7, 2019

    10.0K

    相关概念视频

    CRISPR01:59

    CRISPR

    Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
    57.6K
    CRISPR and crRNAs02:53

    CRISPR and crRNAs

    Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
    The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
    18.8K
    Catalysis02:50

    Catalysis

    The presence of a catalyst affects the rate of a chemical reaction. A catalyst is a substance that can increase the reaction rate without being consumed during the process. A basic comprehension of a catalysts’ role during chemical reactions can be understood from the concept of reaction mechanisms and energy diagrams.
    30.1K
    CRISPR/Cas9 Genome Editing01:28

    CRISPR/Cas9 Genome Editing

    The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
    1.8K
    The Spindle Assembly Checkpoint02:19

    The Spindle Assembly Checkpoint

    The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
    Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
    3.7K
    Introduction to Mechanisms of Enzyme Catalysis01:13

    Introduction to Mechanisms of Enzyme Catalysis

    For many years, scientists thought that enzyme-substrate binding took place in a simple "lock-and-key" fashion. This model stated that the enzyme and substrate fit together perfectly in one instantaneous step. However, current research supports a more refined view scientists call induced fit. The induced-fit model expands upon the lock-and-key model by describing a more dynamic interaction between enzyme and substrate. As the enzyme and substrate come together, their interaction causes...
    10.5K