在直接共发酵过程中对Saccharomyces cerevisiae和Hanseniaspora uvarum进行转录组分析
Yajie Yu1, Desire Nzoyisaba1, Wenke Shi1
1College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China.
Journal of food science
|January 23, 2026
概括
在葡萄酒发酵过程中,Saccharomyces cerevisiae抑制了汉氏菌的卵子生长. 这项研究揭示了酵母相互作用如何改变基因表达,碳代谢和压力反应,影响共发酵动态.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 发酵科学 发酵科学
背景情况:
- 汉氏菌卵子可以提高葡萄酒的质量,但其潜力受到Saccharomyces cerevisiae主导地位的限制.
- 了解酵母-酵母相互作用对于优化发酵过程至关重要.
研究的目的:
- 阐明在直接共发酵 (DCF) 过程中Saccharomyces cerevisiae和Hanseniaspora uvarum之间相互作用的分子机制.
- 在共发酵条件下分析两种酵母物种的基因表达特征和代谢转变.
主要方法:
- 双RNA测序 (RNA-Seq) 用于在DCF期间在两个时间点 (24h和72h) 描述基因表达.
- 在单次发酵 (SF) 和DCF之间对基因表达和代谢途径的比较分析.
主要成果:
- 在DCF期间,Hanseniaspora uvarum经历了显著的生长抑制.
- 麦芽菌 (Saccharomyces cerevisiae) 显示出比H. uvarum更多的差异表达基因 (DEG).
- 在两种物种中都发生了碳流重定向:S. cerevisiae向糖原和H. uvarum向三糖代谢.
- S. cerevisiae面临氧化压力,而H. uvarum经历了与酒精有关的压力.
- 埃尔戈斯特醇代谢途径的差异调节被确定为S. cerevisiae主导的关键因素.
结论:
- 这项研究提供了关于共同发酵期间复杂的酵母-酵母相互作用的见解.
- 转录组数据突出显示了S. cerevisiae和H. uvarum中明显的应激反应和代谢适应.
- 了解这些相互作用为在酒和其他共同发酵系统中管理微生物群落提供了基础.
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