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血液养蚊子的元基因组监测,以评估管理动物环境中的动物感染风险

Mukesh Thakur¹, Stanzin Dolker¹, Avijit Ghosh¹

¹Zoological Survey of India, Kolkata, West Bengal, India.

Vector borne and zoonotic diseases (Larchmont, N.Y.)

|January 23, 2026

概括

血液养的蚊子可以在管理的动物环境中检测动物传染病原体. 这种新的外源监测方法为公共卫生提供了早期预警系统,有助于为新出现的疾病做好准备.

科学领域:

环境科学环境科学
微生物学微生物学
公共卫生公共卫生

背景情况:

媒介传播的动物性传染病对全球的健康构成重大风险,尤其是在人,动物和野生动物的接口处.
目前的监测方法难以在农场和动物园环境中检测早期的动物性疾病溢出.
血液养的蚊子提供了一种非侵入性的环境监测方法 (外来监测),因为它们的宿主养习惯多样化.

研究的目的:

评估使用血液养的蚊子作为生物样本来评估动物传播风险的可行性.
在印度东部的管理动物环境中检测潜在的动物传染病原体的环境DNA特征.

主要方法:

从印度西孟加拉州的畜牧场和动物园收容所收集了185只被血液养的雌性蚊子.
使用线粒体细胞染色体c氧化酶I (COI) 基因测序识别的蚊子物种.
在聚合的蚊子DNA上进行了枪支元基因组测序,并使用Kraken 2和phyloseq.分析了微生物多样性.

主要成果:

确定了6种蚊子物种,包括已知的动物传播媒介.
甲基因组分析揭示了多样化的微生物群落与宿主相关的变异.
检测到潜在的动物传染病原体的基因组片段,如Plasmodium relictum和Babesia bigemina.

结论:

血液养的蚊子是有效的非侵入性样本采集器,用于检测动物传染病原体的环境DNA.
基于蚊子的基因组监测作为一个早期预警和风险检测工具,在一个健康框架内.
将这种方法与诊断相结合,可以提高对新出现的载体传播动物性疾病威胁的准备.

关键词:

一个健康,一个健康.牛津纳米孔测序牛津纳米孔测序转基因组学是指转基因组学.蚊子传播的疾病.野生动物监测监测野生动物的监测.动物传染病原体的病原体

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