在结肠癌细胞中的不对称分裂的可靠评估
Domenico Caudo1,2, Chiara Giannattasio1,3, Simone Scalise1,2
1Center for Life Nano and Neuro Science, Istituto Italiano di Tecnologia, Rome, Italy.
eLife
|January 23, 2026
概括
癌细胞利用不对称的细胞分裂来增加体能. 一种新的流细胞计法准确量化了这些分区波动,有助于理解细胞异质性.
科学领域:
- 细胞生物学 细胞生物学
- 癌症研究 癌症研究
- 生物物理学的生物物理.
背景情况:
- 细胞分裂期间细胞成分的不对称分割对于细胞分化至关重要.
- 癌细胞可能会劫持这种机制以增强表型异质性,可塑性和健康.
- 目前用于量化分区波动的成像方法受到检测细胞分裂的困难的限制.
研究的目的:
- 开发一种通用,准确和高通量方法,用于量化细胞分裂期间细胞元素分区的波动.
- 将这种方法应用于正常和癌症的人体结肠细胞,以评估细胞质分裂的差异.
- 为了验证该方法的准确性与来自活细胞显微镜的直接测量.
主要方法:
- 开发一种新的方法,将高通量流细胞计与理论框架相结合.
- 应用于流细胞测量协议对正常和癌症人类结肠细胞的面板.
- 使用广泛的时隔活细胞激光扫描显微镜测量细胞元素的遗传分数的验证.
主要成果:
- 结肠腺癌细胞表现出明显的细胞质分裂波动,与不对称的细胞大小分裂相关.
- 基于流细胞计的方法显示出与直接显微镜测量遗传小部分的良好一致.
- 该研究成功量化了正常和癌症结肠细胞群中的分区波动.
结论:
- 已经建立了一种基于流细胞计的新,准确和广泛适用的方法来量化分区波动.
- 这种方法可以帮助解释各种生物系统中观察到的表型异质性和可塑性,特别是在癌症中.
- 这些发现突出了不对称分裂在推动结肠癌细胞异质性的作用.
相关概念视频
02:13Determining the Plane of Cell Division
3.8K
Positioning the cell division plane is a critical step during development and cell differentiation, particularly during mitosis when the plane is essential for determining the size of the two daughter cells. The cell division plane is perpendicular to the plane of chromosome segregation, but different types of organisms have different cell division mechanisms to suit their morphology and function.
Animal cells
In animal cells, the cleavage furrow forms along the plane of cell division...
Animal cells
In animal cells, the cleavage furrow forms along the plane of cell division...
3.8K
01:27Molecular Factors Affecting Cell Division
3.9K
Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
3.9K
02:22The Colonization of Land
37.5K
Changes in the environment of the early Earth drove the evolution of organisms. As prokaryotic organisms in the oceans began to photosynthesize, they produced oxygen. Eventually, oxygen saturated the oceans and entered the air, resulting in an increase in atmospheric oxygen concentration, known as the oxygen revolution approximately 2.3 billion years ago. Therefore, organisms that could use oxygen for cellular respiration had an advantage. More than 1.5 years ago, eukaryotic cells and...
37.5K
01:11Rotation of Asymmetric Top
1.5K
By definition, a spherically symmetric body has the same moment of inertia about any axis passing through its center of mass. This situation changes if there is no spherical symmetry. Since most rigid bodies are not spherically symmetric, these require special treatment.
The relationship between the angular momentum of any rigid body and its angular velocity, both of which are vectors, involves the moment of inertia. The moment of inertia is a scalar quantity only for spherically symmetric...
The relationship between the angular momentum of any rigid body and its angular velocity, both of which are vectors, involves the moment of inertia. The moment of inertia is a scalar quantity only for spherically symmetric...
1.5K
01:35Asymmetric Lipid Bilayer
9.7K
Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
9.7K
01:23Functional Divisions of the Nervous System
8.0K
The nervous system, responsible for sensing, integrating, and responding to various stimuli, is divided into the central nervous system (CNS) and the peripheral nervous system (PNS). The PNS has two functional divisions: the sensory or afferent division and the motor or efferent division.
The sensory division transmits information from sensory receptors in the body to the CNS. It provides the CNS with knowledge about somatic senses (such as tactile, thermal, pain, and proprioceptive sensations)...
The sensory division transmits information from sensory receptors in the body to the CNS. It provides the CNS with knowledge about somatic senses (such as tactile, thermal, pain, and proprioceptive sensations)...
8.0K