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自然化合物5,7,8-trimethoxyflavone通过抑制EMT和PI3K/Akt通路来减轻辐射诱导的肺损伤

Cui-Cui Gong¹, Hua-Kang Li¹, Yuan-Zhen Mi¹

¹Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 610032, P.R. China.

International journal of molecular medicine

|January 23, 2026

概括

5,7,8-trimethoxyflavone (HY-N7656) 通过减少炎症和纤维化,有效治疗辐射诱导的肺损伤 (RILI). 它通过PI3K/Akt途径抑制上皮层-介质酶过渡 (EMT),为改善放射治疗安全提供了潜在的策略.

科学领域:

生物医学科学生物医学科学
药理学药理学是指药理学的学科.
在瘤学瘤学.

背景情况:

辐射诱导的肺损伤 (RILI) 是胸部放射治疗的显著剂量限制毒性.
目前对RILI的治疗策略有限,需要开发新的治疗方法.

研究的目的:

为了研究5,7,8-trimethoxyflavone (HY-N7656) 的治疗潜力,一种天然的黄类化合物,在缓解RILI.
阐明HY-N7656作用的基本机制,包括其对上皮质-介质细胞过渡 (EMT) 和关键信号通路的影响.

主要方法:

在体内研究中,使用微型CT,组织病理学,ELISA和西部斑点测试对被辐射的小鼠进行肺损伤的评估.
在体外研究中使用TGF-β刺激的A549细胞通过RT-qPCR和蛋白质表达分析来评估HY-N7656对EMT的影响.
网络药理学和分子对接被用来预测和验证治疗点和信号通路.

主要成果:

在被辐射的小鼠中,HY-N7656显著缓解了肺炎和纤维化,减少了α-平滑肌肉的活性表达.
在A549细胞中,HY-N7656逆转了TGF-β诱导的EMT,恢复了E-cadherin,并降低了间酶体标记物的下调 (N-cadherin,vimentin).
网络药理学确定PI3K/Akt通路是关键目标,实验证实HY-N7656抑制了辐射诱导的PI3K/Akt激活.

结论:

通过抑制炎症,纤维化和EMT,HY-N7656显示出对RILI的显著治疗疗效.
该机制涉及PI3K/Akt信号通路的调制.
HY-N7656代表了RILI的一个有前途的多目标治疗剂,可能提高放射治疗的安全性.

关键词:

5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 6 6 6 6 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 8 8 8 8 8 8 8 8 8 8 8 8 8 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 including including including including including 第七百七十七章没有了 8-trimethoxyflavone的使用情况在PI3K/Akt.表皮细胞-介质细胞过渡.肺部纤维化肺部纤维化辐射引起的肺损伤.

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