基于生命能力的单细胞质谱,用于更全面的代谢分析
Simin Cheng1, Danni Wu1,2, Xinxin Wang3
1Technology Innovation Center of Mass Spectrometry for State Market Regulation, Center for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, China.
Analytical chemistry
|January 23, 2026
概括
我们开发了一种新的质谱法,可以同时测量细胞活力和新陈代谢. 这种基于生存能力的技术准确地区分活细胞和死细胞,揭示了对理解癌症药物反应至关重要的代谢差异.
科学领域:
- 细胞生物学 细胞生物学
- 代谢学 代谢学 代谢学
- 质谱测量质量谱测量
背景情况:
- 细胞活力对细胞功能至关重要,但代谢研究往往忽视活细胞和死细胞之间的差异.
- 这种监督可能导致对生物系统的误解,在基于质谱的研究中.
- 现有的方法缺乏在单细胞水平上同时评估细胞活力和代谢概况的能力.
研究的目的:
- 开发一种高通量,无标签的单细胞质谱技术,其中包含细胞活力信息.
- 同时测量细胞活力和代谢概况,使细胞状态更准确地理解.
- 在质谱数据中识别内在标记物来区分活细胞和死细胞.
主要方法:
- 开发了一种可行性告知单细胞质谱 (ViSCMS) 技术.
- 使用胆 (PC) 34:1用于事件确认和谷氨 (GSH) 作为内在的生命力标志物.
- 验证了ViSCMS与传统的AO/PI染色进行可行性评估.
主要成果:
- 对于生存率,ViSCMS与AO/PI染色具有很高的一致性 (平均偏差为0.33%).
- 在多种细胞系和活力水平上实现了优异的复制性 (SD<3%).
- 成功地亚型化了六种结直肠癌 (CRC) 细胞系,这项壮举只能在考虑细胞活力时实现.
- 在用抗癌药物治疗的HCT116细胞中观察到明显的活力依赖的代谢转变.
结论:
- ViSCMS提供了一种强大而准确的方法,可以同时进行单细胞活力和代谢分析.
- 考虑到细胞活力对于准确的代谢亚型和理解细胞反应至关重要,特别是在癌症研究中.
- 该技术为单细胞层面的瘤细胞适应化疗应激提供了新的见解.
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