排卵分数驱动的猪子宫基因表达和组织学概况的变化
Sonia Heras1,2, Laura Abril-Parreño1,2, Chiara Luongo1
1Departamento de Fisiología, Facultad de Veterinaria, Campus de Excelencia Mare Nostrum Universidad de Murcia, Murcia 30100, Spain.
概括
猪精液血分量的组成会影响母猪子宫基因表达和免疫反应. 包括更多的射精分数会改变子宫环境,影响怀孕早期的结果.
科学领域:
- 生殖生物学 生殖生物学
- 动物科学动物科学
- 基因组学就是基因组学.
背景情况:
- 精液等离子体 (SP) 含有调节女性生殖道的生物活性成分.
- 野猪射精的分化产生了不同组成的部分.
- 这些分量的对子宫环境的累积影响尚不清楚.
研究的目的:
- 研究如何逐步纳入野猪射精分数影响子宫基因表达,细胞增殖和母猪人工授精后免疫反应.
主要方法:
- 母猪被人工授精,精液剂量包括不同的射精分数组合 (FR1,FR2,FR3) 或作为对照.
- 宫组织在雌激素发生后6天被采集用于转录组 (RNA测序) 和组织学分析.
- 基因表达,细胞增殖 (Ki-67) 和免疫细胞透 (CD3+ T细胞) 被量化.
主要成果:
- 与对照组相比,RNA测序揭示了FR2组中差异表达基因 (DEG) 的数量最多,其次是FR3和FR1.
- 大多数DEGs的下调,包括像VNN2.2这样的免疫相关基因.
- 组织学显示,所有AI群体的细胞增殖和T细胞透减少,FR2和FR3群体的分泌表型转变更强.
结论:
- 逐步纳入野猪射精分量的过程引起了明显的子宫转录和组织学反应.
- 精液等离子体的组成显著影响子宫环境.
- 分量特定的SP成分可能在调节母猪早期怀孕结果方面发挥关键作用.
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