渐变扩散条和磁盘扩散与汁微稀释的性能,用于对422个 Staphylococcus aureus 隔离物的莱法林敏感性测试
Yujing Tian1,2, Xiangning He3,4,5, Xue Wu6
1Department of Laboratory Medicine, The Affiliated Taian City Central Hospital of Qingdao University, Taian, China.
Journal of clinical microbiology
|January 23, 2026
概括
使用梯度扩散条带 (GDS) 和磁盘扩散 (DD) 的莱法穆林敏感性测试显示对黄金葡萄球菌的高准确性. 这两种方法都适用于常规实验室,GDS和DD需要确认边界结果以指导莱法林治疗.
科学领域:
- 抗微生物敏感性测试试验 抗微生物敏感性测试
- 临床微生物学 临床微生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 莱法穆林是一种新型的多菌素抗生素,对抗抗甲素耐药黄金葡萄球菌 (MRSA) 具有强烈的活性.
- 微稀释 (BMD) 是敏感性测试的参考方法,但对于常规实验室需要更简单的替代方法.
- 渐变扩散条 (GDS) 和磁盘扩散 (DD) 是对莱法穆林敏感性测试的潜在替代方案.
研究的目的:
- 为了评估GDS和DD的准确性,与BMD相比,用于对黄金葡萄球菌 (Staphylococcus aureus) 的勒法穆林敏感性测试.
- 确定GDS和DD在常规实验室实施中的临床实用性.
- 为适当的莱法穆林治疗和抗菌素耐药性制提供指导.
主要方法:
- 总共测试了422种金黄色葡萄球菌 (256种MSSA和166种MRSA) 的临床分离物.
- 使用BMD (参考),GDS和DD方法进行了敏感性测试.
- 解释遵循CLSI M100-S34断点,计算分类协议 (CA),基本协议 (EA),重大错误 (ME) 和非常重大错误 (VME).
主要成果:
- 莱法穆林对黄金色细菌表现出强烈的活性,BMD的敏感性为98.6%.
- GDS显示100%的CA和92.2%的EA没有VME或ME,但系统地低估了MICs.
- 磁盘扩散达到99.5%的CA,但其VME率超过了CLSI值,这是由于少量的非敏感隔离物.
结论:
- 两种GDS和DD都是准确的,可以在常规实验室中进行勒法穆林敏感性测试.
- 建议在GDS MIC = 0.25 mg/L的情况下确认BMD.
- 任何不敏感的磁盘扩散结果都需要 BMD 验证,以确保适当的勒法林治疗和抗药性.
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