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甲氨酸增强了猪精子的冷生存和抗氧化活性
Malik Ahsan Ali1,2,3,4,5, Yan Zhang1,2,3,4, Weihan Yang1,2,3,4
1State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Reproduction (Cambridge, England)
|January 23, 2026
概括
冷保存通过氧化压力损害了野猪的精子. 恢复由SIRT5和IDH2调节的L-氨酸水平,改善精子运动性,能量,减少活性氧物种 (ROS),提高冷保存精液质量.
科学领域:
- 生殖生物学 生殖生物学
- 精子冷保存 精子冷保存
- 线粒体的新陈代谢
背景情况:
- 冷保存的野猪精子表现出由于活性氧物种 (ROS) 的氧化损伤导致的生育能力降低.
- 在冷保存过程中,线粒体调节器SIRT5和IDH2的下调,影响精子代谢.
- 精子中SIRT5/IDH2失调引起的特定代谢变化尚未完全理解.
研究的目的:
- 为了研究SIRT5和IDH2下调在野猪精子中的代谢后果.
- 为了确定受SIRT5/IDH2失调影响的关键代谢物.
- 评估L-氨酸补充剂减轻冷诱导的损伤和改善精子质量的潜力.
主要方法:
- 在野猪精子中,SIRT5和IDH2通过siRNA电传递被下调.
- 使用液体染色学-并联质谱法 (LC-MS/MS) 确定了差异化代谢物.
- 评估了L-氨酸补充对精子参数的影响,包括运动性,ATP含量,线粒体功能,膜完整性和ROS水平.
主要成果:
- SIRT5/IDH2 knockdown 失调了抗氧化剂通路,并显著降低了L-氨酸的调节.
- 补充80μM的L-氨酸改善了精子的总和渐进性运动性,ATP含量和线粒体功能.
- 补充L-氨酸维持了膜完整性,并在新鲜和冷保存的精子中显著降低了ROS水平.
结论:
- SIRT5/IDH2轴调节L-氨酸代谢,这对于保持精子抗氧化能力至关重要.
- 补充L-氨酸是一种机制驱动的策略,用于在冷保存后提高野猪精子质量.
- 这些发现提供了一个有针对性的方法,通过优化精液质量来提高人工授精 (AI) 的成功率.
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