在小鼠子宫管节的后复合转录造型分析中,可以识别出潜在的精子选择媒介
Nikola Arsić1, María de Dios Castresana1, Eva Pericuesta1
1Department of Animal Reproduction, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) of the Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain.
概括
女生殖道精子选择涉及子宫管节 (UTJ). 研究人员在尿道上皮上发现了新的女性蛋白质LGALS3和ITGA8,这对精子相互作用和性交后的选择至关重要.
科学领域:
- 生殖生物学 生殖生物学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 女生殖道 (FRT) 在合后的精子选择中发挥着关键作用.
- 子宫管节 (UTJ) 是精子选择的关键部位,受精子表面蛋白质的影响.
- 在UTJ与这些精子蛋白相互作用的雌性对应物仍然在很大程度上是未知的.
研究的目的:
- 为了确定与精子相互作用的UTJ表皮上的候选女性蛋白质.
- 在UTJ了解精子选择的分子机制.
- 探索人工生殖技术的潜在应用.
主要方法:
- 在交配后对小鼠FRT组织进行RNA测序 (RNAseq) 分析.
- 生物信息分析以确定UTJ中差异表达的基因.
- 免疫光和西部斑分析验证候选蛋白质LGALS3和ITGA8.
主要成果:
- 确定了240个在UTJ后交配中表达更高的基因.
- 观察到细胞外基因基因的上调,特别是在UTJ.
- 证实了LGALS3和ITGA8在UTJ表皮上的存在和局部化,在交配的雌性中增加了LGALS3.
结论:
- 已确定LGALS3和ITGA8是潜在的相互作用体,它们参与了小鼠尿道中的精子选择.
- 这些发现表明,尿道表皮蛋白在调节精子通道方面起着作用.
- 鉴定出来的蛋白质可能为改善人工生殖技术提供未来的应用.
相关概念视频
Transcription Factors
82.3K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
82.3K
Eukaryotic Transcription Activators
12.6K
Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
12.6K
Transcription
155.8K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
155.8K
Sperm Transport
3.3K
The journey of sperm from its origin to the point of ejaculation begins within the seminiferous tubules of the testis. Here, Sertoli cells produce fluid that propels non-motile sperm through a series of conduits, starting with the straight tubules leading to the rete testis. This interconnected network of tubules acts as the initial pathway for sperm, guiding them into the efferent ductules and then into the epididymis for maturation.
The maturation phase occurs in the epididymis, where sperm...
The maturation phase occurs in the epididymis, where sperm...
3.3K
Transcription Attenuation in Prokaryotes
18.2K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
18.2K
P-N junction
1.2K
A p-n junction is formed when p-type and n-type semiconductor materials are joined together. At the interface of the p-n junction, holes from the p-side and electrons from the n-side begin to diffuse into the opposite sides due to the concentration gradient. This diffusion of carriers leads to a region around the junction where there are no free charge carriers, known as the depletion region. The charge density within the depletion region for the n-side and p-side can be described by the...
1.2K


