纤维细胞生长因子21减轻TM4塞尔托利细胞中FSH诱导的铁亡
Shiming Wang1,2, Yaping Liu1, Nannan Zhang3
1Reproductive Medical Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Reproduction (Cambridge, England)
|January 23, 2026
概括
卵泡刺激激素 (FSH) 在塞尔托利细胞和丸中触发铁亡,一种细胞死亡形式. 纤维细胞生长因子21 (FGF21) 显示出缓解FSH诱导的丸损伤的潜力.
科学领域:
- 生殖生物学 生殖生物学
- 细胞死亡机制 细胞死亡机制
- 内分泌学 在内分泌学.
背景情况:
- 卵泡刺激激素 (FSH) 在男性生殖功能中起着至关重要的作用.
- 了解FSH诱导的丸效应背后的细胞机制至关重要.
- 铁亡是一种受调节的细胞死亡形式,已成为各种病理的重要因素.
研究的目的:
- 调查FSH是否诱导TM4塞尔托利细胞和小鼠丸中的铁亡.
- 为了确定可以减轻FSH诱导的铁亡的潜在因素.
- 探索潜在的分子机制和治疗点.
主要方法:
- 用FSH治疗TM4细胞和小鼠.
- 评估细胞活力,丸组织学和铁亡标志物 (铁,MDA,GSH).
- 对基因和蛋白质表达的分析 (RT-qPCR,西部斑),RNA测序,以及使用铁灭抑制剂 (Fer-1).
- 通过敲击和过度表达来对FGF21的功能验证.
主要成果:
- 在TM4 Sertoli细胞和小鼠丸中,FSH暴露诱导了铁亡.
- 观察到铁灭抑制剂的减少 (SLC7A11,FSP1) 和压力蛋白的增加 (FTH1,HO1).
- FSH增加了铁和MDA,同时减少了GSH,表明氧化应激和铁.
- 铁素-1 (Fer-1) 在TM4细胞中减轻了FSH诱导的铁亡.
- 纤维细胞生长因子21 (FGF21) 被确定为潜在的抑制剂;FGF21的淘汰加剧了铁亡,而FGF21补充剂减轻了它.
结论:
- FSH诱导丸细胞中的铁亡,导致丸损伤.
- FGF21作为一种新型内源性FSH诱导铁灭菌的抑制剂.
- FGF21为管理FSH诱导的丸损伤提供了潜在的治疗标.
关键词:
FGF21FGF21FGF21FGF21FGF21FGF21FGF21FGF21FGF21FGF21FGF21在FSH中,FSH是FSH.铁性化 (ferroptosis) 是一种铁过载是因为铁过载.氧化应激是一种氧化应激.TM4 塞尔托利细胞更多相关视频
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