通过抑制NETs/NF-κB/CXCL3通路,NAC和DNase I协同降低NETs,以减轻严重的急性胰腺炎
Binjie Li1, Pengpeng Liu1, Xuewu Yang1
1Department of General Surgery, Tianjin Medical University General Hospital, Tianjin, 30052, People's Republic of China.
Apoptosis : an international journal on programmed cell death
|January 23, 2026
概括
结合的N-乙半氨酸 (NAC) 和脱氧核糖酶I (DNase I) 疗法有效地减少中性粒细胞外细胞陷 (NETs) 在严重的急性胰腺炎 (SAP). 这种双重方法通过向NETs的形成和降解来缓解胰腺和肺损伤.
科学领域:
- 胃肠病学 胃肠病学
- 免疫学 免疫学 免疫学
- 肺部病理学 肺部病理学
背景情况:
- 中性细胞细胞外陷 (NETs) 显著促进严重急性胰腺炎 (SAP) 的进展,导致胰腺损伤和全身炎症.
- 反应性氧物种 (ROS) 对NETs形成至关重要,对NETs降解构成治疗挑战.
研究的目的:
- 调查N-乙半氨酸 (NAC) 和脱氧核糖酶I (DNase I) 联合治疗对减轻SAP和相关肺损伤的疗效.
- 探索这种双重疗法的潜在分子机制,以抑制NETs的形成和降解.
主要方法:
- 在体外评估NAC对ROS介导NETs形成的影响,以及DNase I对预制NETs的影响.
- 在SAP模型中对早期双NAC和DNase I干预的体内评估,评估NETs负担,中性粒细胞透,细胞亡,炎症性细胞因子和器官损伤.
- 涉及NF-κB激活,CXCL3释放和CXCR2-阳性中性粒细胞招募的机制研究.
主要成果:
- NAC抑制了氧化应激,减少了NETs的形成,而DNase I降解了现有的NETs.
- 结合疗法显示出协同效果,与单一疗法相比,显著降低了NETs负担.
- 在体内,双重干预改善了胰腺炎和肺损伤,通过减少中性粒细胞透,亡和炎症性细胞因子,抑制NF-κB激活和CXCL3 / CXCR2信号传递.
结论:
- 结合NAC和DNase I疗法提供了一个强大的策略,通过同时针对NETs生成和清除来管理SAP.
- 这种协同方法,利用氧化还原调节和酶降解,为严重的急性胰腺炎及其全身并发症提供了一个有前途的治疗途径.
相关概念视频
NF-κB-dependent Signaling Pathway
9.8K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
9.8K
Acute Pancreatitis I: Introduction
1.2K
Pancreatitis is inflammation of the pancreas, an organ located behind the stomach. It can be either acute or chronic.
Acute pancreatitis is characterized by rapid inflammation of the pancreas, often caused by factors like gallstone blockage or excessive alcohol consumption. Chronic pancreatitis, on the other hand, is a slow, progressive inflammation that may result from long-term alcohol abuse, obstructions in the pancreatic duct, or genetic factors.
The causes of acute pancreatitis include:
Acute pancreatitis is characterized by rapid inflammation of the pancreas, often caused by factors like gallstone blockage or excessive alcohol consumption. Chronic pancreatitis, on the other hand, is a slow, progressive inflammation that may result from long-term alcohol abuse, obstructions in the pancreatic duct, or genetic factors.
The causes of acute pancreatitis include:
1.2K
Acute Pancreatitis II: Clinical Manifestations and Management
788
Acute pancreatitis presents a complex medical emergency characterized by rapid onset inflammation of the pancreas, demanding timely diagnosis and management to prevent complications. The condition primarily manifests through severe upper abdominal pain that often radiates to the back. This pain intensifies following the consumption of fatty foods. Accompanying symptoms such as nausea, vomiting, abdominal distention, fever, dyspnea, cyanosis, and jaundice can vary in intensity but significantly...
788
Transcription Attenuation in Prokaryotes
18.2K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
18.2K
C4 Pathway and CAM
48.9K
Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
C4 Pathway
The C4 pathway is used by plants such as...
48.9K
Reducing Line Loss
371
In a three-phase circuit, line loss is an indicator of energy dissipated as heat due to the resistance of transmission lines. To address this, incorporating transformers into the system—a step-up transformer at the source and a step-down transformer at the load—is a strategic solution. Two three-phase transformers are introduced to improve this.
With a step-up transformer at the source, the voltage is increased, thereby reducing the current in the transmission lines since power loss in...
With a step-up transformer at the source, the voltage is increased, thereby reducing the current in the transmission lines since power loss in...
371


