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The “tree of life” describes the evolution of life and the evolutionary relationships between organisms. The root of the tree is the common ancestor to all life on Earth. All other species radiate from this point, much like the branches of a tree. The numerous tips of these branches on the tree of life represent every living, or extant, species. Extinct species, which are species that no longer exist, can be found towards the center of the tree. Currently, these organisms, both...
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布斯科基尼:一个BUSCO杆化了家族基因树建设者.

John Webster1, Toni A Chapman2

  • 1NSW Department of Primary Industries and Regional Development, Sydney, NSW, Australia. john.webster@dpird.nsw.gov.au.

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概括

布斯科基尼是一种新的工具,可以使用BUSCO衍生基因自动创建家族遗传树. 这种方法从全基因组数据提供了强大的进化见解,特别是对于真菌.

关键词:
自动化自动化自动化布斯科 (Busco) 是一个古树.生物信息学是一种生物信息学.菌的真菌是一种真菌.人类遗传学学.遗传学上,这是一个很大的问题.管道管道 管道管道管道再组合过器进行过.纳税学是一种分类学.

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科学领域:

  • 进化生物学 进化生物学
  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.

背景情况:

  • 遗传树对于理解进化关系至关重要.
  • 单基因的基因组系对复杂的基因组来说可能不足.
  • 产生连接的基因对齐对像真菌这样的标注较差的基因组来说具有挑战性.

研究的目的:

  • 介绍Buscogeny,这是一个开源工具,用于自动化遗传树的构建.
  • 为了从全基因组数据中进行可靠的进化推断.
  • 为了简化研究人员与多样化或不完整的基因组数据集工作的过程.

主要方法:

  • 通过使用BUSCO衍生的单拷贝正义词来自动构建连接的基因对齐.
  • 整合基因组质量评估,正义提取和对齐.
  • 包含差距和重组过,然后进行自动化家族遗传推断.

主要成果:

  • 在细菌和真菌基因组的遗传学分析中证明了布斯科基尼的实用性.
  • 在复杂的真菌群体中成功应用,如具有可变基因组完整性的Alternaria.
  • 精简和可重复的工作流程用于基于BUSCO的基因推理.

结论:

  • 布斯科基尼从全基因组数据中提供了强大的进化见解.
  • 该工具提高了跨学科研究人员对遗传学推理的可访问性.
  • 能够更准确地解决进化关系,特别是对于具有挑战性的种类.