莲花:一种低成本的时隔自动成像系统,用于微生物殖民地或生物膜发育的时空分析
Ryunosuke Sakai1, Yifan Zhao1, Martin Robert1
1Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan.
PloS one
|January 23, 2026
概括
我们开发了LOTUS,这是一个廉价的,使用3D打印的自动成像系统. 这种开源平台可以实现微生物研究和教育的并行,时间缩短的实时成像.
科学领域:
- 生命科学 生命科学
- 微生物学 微生物学
- 生物工程是生物工程.
背景情况:
- 开放科学正在推进低成本技术,如单板计算机和3D打印机.
- 在生命科学研究和教育中,对可访问,负担得起的成像系统的需求越来越大.
- 现有的成像解决方案往往缺乏成像模式多样性和足够的吞吐量.
研究的目的:
- 开发一种低成本的自动成像系统,用于微生物发育的时空分析.
- 为监测殖民地或生物膜生长和基因表达提供一个多功能平台.
- 为了提高自动化时隔实时成像的可访问性,用于研究和教育.
主要方法:
- 设计和制造了LOTUS,一个使用3D打印组件的550美元的自动成像系统.
- 集成的机动样本定位与可互换的LED光源和光学过器.
- 使用了四种成像模式:明亮场转光,明亮场光和双色光.
主要成果:
- 莲花在3天内表现出稳定且可重复的定时和定位精度.
- 实现了均的LED照明,并捕获了适合半定量分析的图像.
- 成功成像了大肠杆菌生物膜,在5天的时间内对安培治疗后跟踪光动态.
结论:
- 莲花是一个具有成本效益的,多功能平台,用于并行监测微生物殖民地/生物膜的发展和记者基因表达.
- LOTUS的开源性质使得自动化时差实时成像可用于各种研究和教育应用.
- 该系统解决了微生物研究的负担得起,多模式成像方面的差距.
相关概念视频
Biofilms
1.3K
Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...
1.3K
Noncompartmental Analysis: Mean Residence Time
598
According to statistical moment theory, mean residence time (MRT) is an important measure in pharmacokinetics. MRT can be defined as the expected mean of a probability density function distribution. It provides valuable insights into drug disposition in the body.
After the administration of a drug through intravenous bolus injection, the drug molecules are distributed throughout the body and remain there for varying periods. The MRT represents the average time these drug molecules stay in the...
After the administration of a drug through intravenous bolus injection, the drug molecules are distributed throughout the body and remain there for varying periods. The MRT represents the average time these drug molecules stay in the...
598
Microbial Morphologies
2.0K
Bacterial and archaeal cells exhibit remarkable diversity in shape and structure, critical in their adaptability and functionality. Among bacteria, the most commonly observed shapes include cocci and bacilli. Cocci are spherical and may exist singly or in groupings such as pairs (diplococci), chains (streptococci), clusters (staphylococci), or tetrads. Bacilli, in contrast, are rod-shaped and can also occur as single cells, in pairs, or chains, depending on their environmental and genetic...
2.0K
Microbial Fermentation
1.4K
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
1.4K
Microbial Nutrition
1.1K
Organisms exhibit remarkable metabolic diversity, categorized based on how they acquire energy and carbon. These strategies enable survival in various ecological niches and are essential for maintaining energy flow and nutrient cycling within ecosystems.Energy and Carbon SourcesOrganisms are classified as phototrophs or chemotrophs based on energy acquisition. Phototrophs use light as their energy source, while chemotrophs rely on oxidizing chemical compounds. Further differentiation arises...
1.1K
Microbial Classification System
1.1K
Classification is the process of organizing organisms into hierarchically inclusive groups based on their phenotypic similarities or evolutionary relationships. A species comprises one or more strains, and closely related species are grouped into genera. Genera are further classified into families, families into orders, orders into classes, and so forth, up to the domain level, which is the broadest taxonomic rank derived from a combination of phenotypic and genotypic data.The nomenclature of...
1.1K


