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混塑性FVB/N-mt129S6/SvEvTac小鼠:癌症研究的一个新工具
Artiom Gruzdev1, Wendy N Jefferson1, Thomas B Hagler1
1Reproductive & Developmental Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Durham, North Carolina, United States of America.
由于线粒体基因多态化,FVB/N小鼠表现出加速的子宫内膜癌和增加的活性氧物种. 结合性小鼠显示出更高的癌症等级,但改善了胚胎干细胞的产生,有助于癌症研究.
科学领域:
- 生殖生物学 生殖生物学
- 线粒体遗传学线粒体遗传学
- 癌症研究 癌症研究
背景情况:
- 在癌症研究中,FVB/N小鼠被广泛使用.
- 在受到雌激素暴露后,它们表现出加速的子宫内膜癌.
- 一个mt-Atp8基因多态性增加了反应性氧物种 (ROS) 产量.
研究的目的:
- 研究mt-Atp8多态性在子宫内膜癌中的作用.
- 评估线粒体基因组对癌症发育和胚胎干细胞生成的影响.
主要方法:
- 产生了具有FVB/N核和129S6/SvEvTac线粒体基因组的共塑性FVB/N-mt129小鼠.
- 暴露于FVB/N和FVB/N-mt129小鼠中的二甲基stilbestrol (DES).
- 对比子宫内膜癌的发病率,等级和胚胎干细胞生成能力.
主要成果:
- 在FVB/N和FVB/N-mt129小鼠之间癌症发病率没有差异.
- 结合性FVB/N-mt129小鼠表现出更高的癌症等级.
- FVB/N-mt129芽细胞很容易产生支持基因编辑的多能胚胎干细胞.
结论:
- 线粒体基因组会影响子宫内膜癌等级,但不会影响FVB/N小鼠的发病率.
- 结合性FVB/N-mt129小鼠对于研究癌症表型非常有价值.
- 这些小鼠有助于产生具有FVB/N核基因组的生殖系能力的胚胎干细胞.
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