来自Pseudomonas aeruginosa的一种定数感知分子诱导了共感染病原体的防御性多细胞性
Stefan Katharios-Lanwermeyer1,2, Tiffany M Zarrella1,2, Marshall Godsil1
1Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
概括
在感知Pseudomonas aeruginosa定数感应分子时,Stenotrophomonas maltophilia会形成保护性聚合物. 这种细菌聚合增强了对共感染病原体的生存能力,揭示了一种新的物种间交流机制.
科学领域:
- 微生物学 微生物学
- 细菌之间的相互作用
- 跨物种交流 跨物种沟通
背景情况:
- 微生物在多微生物群落中相互作用,在对分泌的分子的反应中改变行为.
- 跨物种沟通和反应的机制仍未得到充分探索,特别是在共感染中.
研究的目的:
- 调查Stenotrophomonas maltophilia和Pseudomonas aeruginosa之间相互作用的情况.
- 阐明了由P. aeruginosa引起的S. maltophilia聚合背后的分子机制.
主要方法:
- 实验进化以确定聚合的遗传基础.
- 非目标代谢学和目标验证以识别信号分子.
- 共同培养实验以评估生存和聚合.
主要成果:
- 在暴露于P. aeruginosa分泌的因子时,S. maltophilia会形成多细胞聚合物.
- 两种S. maltophilia细胞上的膜对于聚合至关重要.
- 伪omonas类信号 (PQS) 直接诱导S. maltophilia聚合,并提高了生存率.
- 由PQS诱导的聚合是多种细菌物种的保存反应.
结论:
- 在P. aeruginosa中,定数感应分子PQS作为S. maltophilia的"危险"信号.
- 诱导的多细胞聚合为S. maltophilia在共感染中提供了生存优势.
- 鉴定了由PQS介导的物种间交流和保护性反应的一般机制.
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