宏观植物投影成像 (MAPPI):一个开放的,可扩展的平台,用于整个植物的光实时成像
Giorgia Tortora1, Bianca Maria Orlando Marchesano2, Stefano Buratti2,3
1Department of Physics, Politecnico di Milano, Piazza Leonardo da Vinci, 20133 Milan, Italy.
Science advances
|January 23, 2026
概括
宏观植物投影成像 (MAPPI) 是一个新的,实惠的平台,用于实时,整个植物的光成像. 这个工具可视化了土壤中生长的植物的系统信号,有助于植物通信研究.
科学领域:
- 植物生物学 植物生物学
- 分子成像学分子成像学
- 系统生物学 系统生物学
背景情况:
- 在整个植物中实体监测分子信号对于了解植物反应至关重要.
- 目前的光显微镜工具仅限于小型模型生物和专用设备.
- 需要对更大,土壤中生长的植物进行实时可访问的成像.
研究的目的:
- 介绍MAcro植物投影成像 (MAPPI),一个开源的,低成本的光成像平台.
- 启用宽视野,双投影成像,以可视化土壤中生长的植物中的系统信号.
- 提供一个可扩展的工具来剖析不同植物物种的信号传播和通信.
主要方法:
- 开发了宏观植物投影成像 (MAPPI),这是一个模块化,低成本的光成像平台.
- 利用双投影成像来实现光记者的广视场可视化.
- 在各种压力条件下的成年植物 *Nicotiana benthamiana* 中追踪和l-谷氨酸的动态.
主要成果:
- 证明了MAPPI在土壤中生长的植物中实时可视化系统信号的能力.
- 揭示了发育调节的长距离波,由伤害,烧伤和潜水引发.
- 观察到对刺激的双向射出到根和根到射出信号.
结论:
- MAPPI实现了全植物功能成像的民主化,使其能够超越模型生物和幼苗.
- 该平台促进了对系统信号传播,应激适应和器官间通信的研究.
- MAPPI是一个可扩展的工具,用于在模型和非模型物种中推进植物生物学研究.
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