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4D打印的ELISA针面板计数器使用4D打印方法.

Hsiao-Chu Chiu1, Cheng-Kuan Su1

  • 1Department of Chemistry, National Chung Hsing University, Taichung City, 402227, Taiwan, ROC.

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概括
此摘要是机器生成的。

使用数字光处理四维打印 (4DP) 的新型传感器可以在现场检测氨基醇 (CAP) 抗生素. 这种氧化还原反应装置为食品安全分析提供了无染色体的方法.

关键词:
胺尼科尔 (Chloramphenicol) 是一种化学物质.与酶相关的免疫吸收剂测定.食品化学 食品化学 食品化学四维打印是一种四维打印.具有氧化回应性的材料.

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科学领域:

  • 分析化学 分析化学
  • 材料科学 材料科学 材料科学
  • 生物技术是生物技术.

背景情况:

  • 与酶相关的免疫吸收试验 (ELISA) 对于检测食品中的抗生素至关重要.
  • 目前的ELISA方法通常需要专门的设备,并且缺乏现场适用性.
  • 在食品安全方面需要快速的现场抗生素检测方法.

研究的目的:

  • 开发一个现场的,无染色体的ELISA用于氨基醇 (CAP) 检测.
  • 使用数字光处理四维打印 (4DP) 来制造一种新型传感器件.
  • 为了创建一个用于定量抗生素分析的氧化还原反应传感器.

主要方法:

  • 使用4DP和EDT结合树脂制造带有氧化回应针的面板计数器.
  • 针对竞争力ELISA的抗CAP抗体涂层的优化.
  • 利用CAP和CAP-HRP之间的竞争来诱导依赖于CAP度的针曲.

主要成果:

  • 传感器表现出依赖过氧化的曲,原因是氧化还原反应性胀.
  • 为CAP.达到2.5 pg mL-1的方法检测极限.
  • 在各种食物矩阵 (鱼,,肉,蜂蜜,牛奶) 中对传统ELISA验证了该方法.

结论:

  • 4DP技术可以制造智能氧化回应感应器.
  • 开发的方法为现场定量化学分析提供了可靠和适用的方法.
  • 这一进步增强了传统的免疫化学测定,以改善食品安全监测.