虫对土壤病毒相关的ARG和长期田间作物系统中耐药性表型的影响
Ying Ding1, Bo-Wen Liu2, Di Wu3
1Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China; State Key Laboratory of Regional and Urban Ecology, Ningbo Observation and Research Station, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China; Zhejiang Key Laboratory of Pollution Control for Port-Petrochemical Industry, CAS Haixi Industrial Technology Innovation Center in Beilun, Ningbo 315830, China.
Journal of hazardous materials
|January 23, 2026
概括
长期调节土壤抗体,在两年内抑制高风险抗生素耐药性基因 (ARG) 和毒性因子基因 (VFG). 活动稳定了微生物群落和病毒宿主网络,为耐药性风险管理提供了见解.
科学领域:
- 土壤生态学 土壤生态学
- 微生物生态学 微生物生态学
- 环境微生物学 环境微生物学
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 在田野条件下,地对土壤抗体的长期影响尚不清楚.
- 土壤抵抗体,包括抗生素耐药性基因 (ARG) 和毒性因子基因 (VFG),对环境和公共健康至关重要.
- 了解虫引入等生态干预如何影响这些抗体对抗性风险管理至关重要.
研究的目的:
- 通过在两年内一次性地添加,研究土壤抗体的持久调节.
- 分析土活动对ARG,VFG,微生物群落结构以及根球和散土中的病毒宿主相互作用的影响.
- 阐明土引入后表型和基因型耐药性变化的时间动态.
主要方法:
- 在长期实地实验中进行了一项为期两年的玉米-小麦旋转研究.
- 综合的元基因组和病毒基因组分析被用来评估土壤微生物和病毒群落.
- 进行了表型耐药性测试,并对ARG和VFG进行了基因型分析.
主要成果:
- 与散装土壤相比,树叶球土壤始终显示出更高水平的ARG和VFG.
- 长期地活动显著抑制了与病毒相关的ARG/VFG和高风险ARG,特别是在散装土壤中.
- 现型耐药性在第一年增加,没有直接的基因型变化,在第二年出现基因型变化.
- 虫促进了向K-战略特征的转变,增加了溶解性,并稳定了病毒-宿主网络.
- 考多病毒菌体将ARG与营养循环细菌联系起来,地增强了这些跨王国相互作用.
结论:
- 对土壤微生物的适应和耐药性动态产生显著的长期调节作用.
- 虫引入可以抑制高风险抗生素耐药性基因和毒性因子的传播.
- 这些发现支持虫在"一个健康"框架内的耐药性风险管理中的作用.
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