阿斯加德古老的ESCRT蛋白质定位到真核细胞细胞结构中
Kishore Babu Naripogu1, Yee Han Tee2, Yosuke Senju1
1Research Institute for Interdisciplinary Science (RIIS), Okayama University, Okayama, 700-8530, Japan.
Biochemical and biophysical research communications
|January 23, 2026
概括
古老的考古ESCRT蛋白质,对于真核细胞结构至关重要,被招募到人类细胞站点. 这表明,在真核生成过程中,在真核细胞膜之前存在着保存的分子相互作用,有助于细胞复杂性.
科学领域:
- 细胞生物学 细胞生物学
- 进化生物学 进化生物学
- 生物化学 生物化学
背景情况:
- 细胞内膜系统在细胞生成过程中出现,但细胞前蛋白质的适应仍然不太了解.
- 亚斯加德古生物,是真核生物的最亲近的原生生物亲属,具有内体排序复合物所需的运输 (ESCRT) 蛋白质的同类物,对真核生物的膜重塑至关重要.
研究的目的:
- 在真核生物环境中研究来自阿斯加德考古物 *Promethearchaeum syntrophicum* (MK-D1) 的ESCRT同类的功能和本地化.
- 探索ESCRT介导的膜重塑的进化起源及其在真核生成中的作用.
主要方法:
- 在人类细胞中表达ESCRT-II和ESCRT-III同类物,VSP4和ubiquitin,来自P. syntrophicum (MK-D1).
- 使用显微镜分析这些古老蛋白质在人体细胞内的局部化模式.
主要成果:
- 在人类细胞中表达时,考古ESCRT蛋白质 (VSP4和ubiquitin) 被招募到特定的结构中.
- 这些蛋白质定位在正规的真核细胞ESCRT招募部位,包括中体和中心体,尽管这些结构在MK-D1.1中不存在.
- 这种保存的局部化表明分子相互作用早于真核生物的内部膜和细胞结构.
结论:
- 这些发现支持一个模型,在这种模型中,祖先的蛋白质机器在真核生成过程中被重新使用.
- 这种重新定位使现有的ESCRT功能和网络更容易被整合到新的细胞架构中,从而增加了真核生物的复杂性.
相关概念视频
Replication in Eukaryotes
204.2K
Overview
204.2K
The Eukaryotic Promoter Region
18.7K
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences. The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
18.7K
Protein and Protein Structure
87.1K
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme...
A protein's shape is critical to its function. For example, an enzyme...
87.1K
Eukaryotic RNA Polymerases
26.8K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
All three eukaryotic RNAPs require specific transcription factors, of which the...
26.8K
Structural Protein Function
29.8K
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...
29.8K
Additional Subnuclear Structures
5.3K
The eukaryotic nucleus is a double membrane-bound organelle that contains nearly all of the cell’s genetic material in the form of chromosomes. It is rightly called the “brain” of the cell as it shoulders the responsibility of responding to various physiological processes, stress, altered metabolic conditions, and other cellular signals.
The nucleus contains many membrane-less subnuclear organelles or nuclear bodies, such as nucleoli, Cajal bodies, speckles,...
The nucleus contains many membrane-less subnuclear organelles or nuclear bodies, such as nucleoli, Cajal bodies, speckles,...
5.3K


