全球APSES转录因子PeStuA调节了Penicillium expansum的生长,发育,应激反应,疏水性,病原蛋白生物合成和病原性
Yanling Wang1, Yurui Wu1, Qianrun Liang1
1School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, 730050, China.
International journal of food microbiology
|January 23, 2026
概括
APSES转录因子PeStuA对于Penicillium expansum的生长,致病性和病原体毒素的产生至关重要. 它的缺失显著损害了真菌的发展,并减少了果中蓝色菌的腐烂.
科学领域:
- 菌类学 菌类学是指菌类学.
- 植物病理学 植物病理学
- 分子生物学分子生物学
背景情况:
- 虫 (Penicillium expansum) 导致蓝色菌的腐烂,并产生真菌毒素 (patulin),导致经济损失和食品安全问题.
- APSES转录因子调节真菌发育,但它们在P. expansum中的具体作用在很大程度上是未知的.
研究的目的:
- 为了研究APSES转录因子PeStuA在Penicillium expansum中的功能.
- 了解PeStuA在真菌生长,应激反应,致病性和真菌毒素生产中的作用.
主要方法:
- 在P. expansum. 中对APSES家族成员进行查.
- 构建和表型分析PeStuA淘汰和补充菌株.
- 果的致病性测试和使用HPLC-UV.的病原蛋白量化.
- RNA测序 (RNA-seq) 分析以确定差异表达的基因.
主要成果:
- 删除PeStuA显著抑制了P. expansum的生长,子的形成和水性.
- PeStuA突变体对透和细胞壁完整性应激的敏感性增加.
- 致病性测试显示,果中病变直径减少,果实变软,果被突变病毒感染.
- PeStuA的淘汰会导致帕图林生产的完全丧失和相关生物合成基因的下调.
- RNA-seq在ΔPeStuA中鉴定了1826个差异表达的基因,影响了各种代谢和信号通路.
结论:
- 在P. expansum. 中,PeStuA充当协调生长,应激反应,疏水性,致病性和二次新陈代谢的中央调节者.
- 这些发现为P. expansum的生物学和收获后疾病控制的潜在目标提供了洞察力.
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