通过酵母酶固定在双层功能化石墨烯纳米纤维上的酵母酶固定来增强银酸酸的生产和净化
Aicha Chenafa1, Yangyang Gu2, Yunping Zhu3
1Key Laboratory of Green Manufacturing and Biosynthesis of Food Bioactive Substances, China General Chamber of Commerce, School of Food and Health, Beijing Technology and Business University, Beijing, 100048, China; Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing, 100048, China.
International journal of biological macromolecules
|January 23, 2026
概括
这项研究开发了一种新型纳米材料,用于使用固定酵母和β-Galactosidase增强银河酸 (GOS) 生产. 吸附方法实现了高产量和纯度,显示了工业GOS合成的前景.
科学领域:
- 生物技术是生物技术.
- 材料科学 材料科学 材料科学
- 酶工程是什么? 酶工程是什么?
背景情况:
- галактоолигосахарид (GOS) 是具有显著健康益处的益生菌.
- 有效和可扩展的GOS合成仍然是一个挑战.
- 酶固定对于提高酶的稳定性和可重复使用性至关重要.
研究的目的:
- 研究Saccharomyces cerevisiae酵母和β-Galactosidase (GAL) 在双层聚烯/功能化石墨烯氧化物上的固定.
- 为了比较吸附和封装固定技术的效率.
- 评估固定对酶活性,稳定性和GOS合成的影响.
主要方法:
- 用吸附和封装在聚乙烯/石墨烯氧化物纳米材料上将酵母细胞和GAL固定.
- 使用XRD,FTIR和SEM进行了结构性表征.
- 在一个两阶段的过程中,使用固定 GAL.GOS 的合成进行了评估.
- 在各种温度和pH条件下测试了酶稳定性.
主要成果:
- 吸附方法产生了99%的酵母不动化和97%的酶不动化.
- 通过吸附的固定对酵母细胞和纤维的结构性质的影响最小.
- 固定AL在各种温度和pH值范围内表现出极好的稳定性.
- 使用固定AL的两阶段工艺实现了67%的GOS产量,单糖污染率低.
结论:
- 固定酵母纳米材料为高效的GOS生产提供了一个有希望的方法.
- 在这种系统中,吸附技术对酵母和酵素不移的优越.
- 这种方法促进了可扩展和高纯度的GOS合成,减少了单糖污染.
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