细胞循环调节单极螺旋一结合剂 (LdMOB1) 蛋白质的晶体结构:识别潜在的不同酸化位
Surendra Saw1, Pankaj Singh Parihar1, Amogh A Sahasrabuddhe2
1Biochemistry and Structural Biology Division, CSIR-Central Drug Research Institute, Lucknow, 226031, Uttar Pradesh, India.
International journal of biological macromolecules
|January 23, 2026
概括
单极螺旋 一个结合剂 (MOB1) 蛋白调节细胞分裂. 研究人员阐明了Leishmania donovani MOB1结构,揭示了其功能至关重要的独特酸化位点.
科学领域:
- 分子生物学分子生物学
- 结构生物学 结构生物学
- 细胞生物学 细胞生物学
背景情况:
- 单极螺旋一个绑定剂 (MOB1) 蛋白质是关键的细胞循环调节剂和瘤抑制剂.
- 在Hippo信号通路中,MOB1充当了适配器,将MST1/2和LATS1激酶连接起来.
- 观察到MOB蛋白的进化保护,N端区域和酸化位点的变化.
研究的目的:
- 为了确定Leishmania donovani MOB1 (LdMOB1) 的晶体结构.
- 调查LdMOB1.1的酸化状态和潜在的酸化部位.
主要方法:
- 使用X射线晶体学以1.3 Å分辨率确定LdMOB1的结构.
- 对本地和T14A突变LdMOB1.1进行了酸化试验.
- 进行了计算型蛋白质-蛋白质相互作用研究.
主要成果:
- LdMOB1的晶体结构显示出一个紧的球形形状,核心结构保留,但N和C末端可变.
- 酸化试验表明LdMOB1经历可逆酸化.
- 在Ser 167和Thr 170确定了潜在的酸化部位,与人类的MOB1部位不同.
结论:
- LdMOB1与同类具有结构上的相似性,但具有独特的酸化位点.
- 已确定的酸化位 (Ser 167, Thr 170) 与人类的MOB1不同,可能参与LdMOB1调节.
- 这些发现有助于理解跨物种的MOB1蛋白功能和调节.
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