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相关概念视频

The de Broglie Wavelength02:32

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In the macroscopic world, objects that are large enough to be seen by the naked eye follow the rules of classical physics. A billiard ball moving on a table will behave like a particle; it will continue traveling in a straight line unless it collides with another ball, or it is acted on by some other force, such as friction. The ball has a well-defined position and velocity or well-defined momentum, p = mv, which is defined by mass m and velocity v at any given moment. This is the typical...
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A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
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During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
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相关实验视频

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Three-dimensional Optical-resolution Photoacoustic Microscopy
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多光谱激光扫描光声学显微镜用SRS生成的波长进行皮肤染色体表征.

Amir Khansari1, Seyed Mohsen Ranjbaran1, Mohsin Zafar1

  • 1The Richard and Loan Hill Department of Biomedical Engineering, University of Illinois at Chicago, Chicago, USA.

Journal of biophotonics
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概括

这项研究引入了一种新的多光谱激光扫描光声学显微镜 (MS-LS-PAM) 系统,用于无标签的皮肤成像. 先进的平台准确地可视化皮肤部件和氧气和,没有对比剂.

关键词:
交叉相关性 交叉相关性深度学习是一种深度学习.多光谱光声显微镜学摄影声学 摄影声学信号的分解信号的分解

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科学领域:

  • 生物医学光学 生物医学光学
  • 医疗成像医学成像
  • 皮肤病学 皮肤病学

背景情况:

  • 光声显微镜 (PAM) 提供了无标签的成像功能.
  • 在皮肤中区分内生染色体需要精确的光谱分辨率.
  • 目前的方法可能需要外源的对比剂或缺乏定量分析.

研究的目的:

  • 开发和验证一个单一的源,多光谱激光扫描光声学显微镜 (MS-LS-PAM) 平台.
  • 为了实现内源性皮肤染色体的高分辨率,无标签成像.
  • 进行染色体分布和氧和量的定量分析.

主要方法:

  • 集成基于激发式拉曼散射 (SRS) 的波长生成,用于可调的激发 (532571 nm).
  • 使用一个紧的,基于纤维的光源来分辨氧血球蛋白 (HbO2),脱氧血球蛋白 (Hb),黑色素和原蛋白.
  • 使用非负最小平方 (NNLS) 进行光谱分离和定量分析.

主要成果:

  • 在体内内生长的染色体的精确光谱分离.
  • 空间分辨率的血液氧和 (sO2) 的定量映射.
  • 在B6和SKH1小鼠模型中清晰可视化原和黑色素结构.

结论:

  • 开发的MS-LS-PAM平台提供高分辨率,无标签的皮肤成像.
  • 该系统能够精确量化色谱分布和生理参数,如sO2.
  • 这项技术有可能用于先进的皮肤病诊断和研究.