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Updated: Jan 25, 2026

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
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超越结合亲和关系:详细描述蛋白质 - 连接物相互作用与时间解析的FRET

Huan Liu1,2,3, Fei Yang1,2, Wenjing Li1,2

  • 1Key Laboratory of Marine Drug, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.

Analytical chemistry
|January 23, 2026
PubMed
概括

一个新的时间解析的Förster共振能量传输 (TR-FRET) 平台提供了详细的洞察力E3联酶连接体相互作用. 这种方法有助于设计用于向蛋白质降解疗法的新型配体.

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Ligand Binding Sites02:40

Ligand Binding Sites

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Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
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Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
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The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium:
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Overview
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