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开发一种微小肠道内置注射技术,用于向的宏分子输送和在小鼠体内功能研究

Yawen Lai¹, Xintao Zhang¹, Tingting Luo¹

¹Guangdong Provincial Key Laboratory of Proteomics, Department of Pathophysiology, School of Basic Medical Science, Southern Medical University, Guangzhou, China.

Animal models and experimental medicine

|January 23, 2026

概括

一种新的最小侵入性技术允许直接将大分子输送到小肠,克服降解问题. 这种方法可以进行精确的肠道研究和药物输送评估.

科学领域:

胃肠病学胃肠病学
手术创新在外科创新.
分子输送系统分子输送系统

背景情况:

生物大分子的向输送到小肠被胃环境中的降解所阻碍.
现有的方法,如口腔测量,在传递敏感生物分子方面效率低下.
开发用于直接肠道输送的新策略对于治疗进展至关重要.

研究的目的:

引入和验证一种最小侵入性,现场注射技术,用于将局部化宏分子输送到小鼠小肠.
评估这种新型输送方法的安全性和有效性.
证明其对研究应用的有用性,包括药物输送和基因疗法.

主要方法:

一种微创手术方法,涉及直接的十二指肠注射到小肠.
在 72 小时内对生理参数,炎症标志物,肝功能和肠道完整性进行术后监测.
使用单细胞RNA测序对功能蛋白TAT-EGFP (Tat蛋白与增强的绿色光蛋白融合) 的组织病理学分析和输送评估.

主要成果:

该技术被证明是安全的,只有暂时的,麻醉相关的副作用,对生理参数或肠道完整性没有重大影响.
成功地将TAT-EGFP输送到肠道上皮细胞实现了,其表现优于口服 gavage.
单细胞RNA测序揭示了在高流动性组的第1框中,在上皮细胞中特定的炎症基因表达模式.

结论:

这种微创的技术在生理环境下提供精确,耐降解的宏分子到小肠的输送.
它为肠道研究提供了一个多功能平台,包括药物输送,基因疗法评估和宿主微生物群相互作用.
该方法在某些研究应用中超越了传统的方法,如口腔和有机体培养.

关键词:

动物模型动物模型药物通过肠道输送.蛋白质的功能蛋白质的功能

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